{"product_id":"bim-antibody-bcl-2-like-protein-11-bha17136091","title":"Bim Antibody \/ Bcl-2-like protein 11","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBim Antibody \/ Bcl-2-like protein 11 is a anti-BCL-2-LIKE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCL-2-LIKE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBim antibody detects Bcl-2-like protein 11, a pro-apoptotic member of the Bcl-2 family that promotes programmed cell death through mitochondrial outer membrane permeabilization. The UniProt recommended name is Bcl-2-like protein 11 (BCL2L11). Also known as Bim, this BH3-only protein is a critical initiator of apoptosis in response to cellular stress, cytokine withdrawal, or DNA damage.\u003cbr\u003e\u003cbr\u003eFunctionally, Bim antibody identifies a 198-amino-acid cytoplasmic protein that binds anti-apoptotic Bcl-2 proteins (such as BCL2, BCL-XL, and MCL1), releasing pro-apoptotic effectors BAX and BAK to trigger mitochondrial cytochrome c release and caspase activation. Bim exists in several isoforms (BimEL, BimL, and BimS) generated by alternative splicing, which modulate apoptosis sensitivity across cell types.\u003cbr\u003e\u003cbr\u003eThe BCL2L11 gene is located on chromosome 2q13 and is expressed in lymphoid, neuronal, and epithelial tissues. Its expression is tightly regulated by survival pathways including PI3K\/AKT and MAPK signaling, which control phosphorylation and proteasomal degradation of Bim.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of Bim contributes to cancer, autoimmune diseases, and neurodegeneration. Reduced Bim expression confers resistance to apoptosis in tumor cells, while overexpression causes excessive neuronal death. Research using Bim antibody supports studies in cell death signaling, oncology, and stress-induced apoptosis.\u003cbr\u003e\u003cbr\u003eBim antibody is validated for western blotting, immunohistochemistry, and apoptosis assays to detect Bcl-2 family proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Bcl-2-like protein 11 contains a BH3 domain required for binding to anti-apoptotic Bcl-2 members and activating the intrinsic apoptotic pathway. This antibody supports exploration of Bim's role in stress-induced mitochondrial apoptosis and cellular homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCL-2-LIKE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCL-2-LIKE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCL-2-LIKE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCL-2-LIKE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCL-2-LIKE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314809197,"sku":"FY13189","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f9578ac1-887a-40c0-ab29-9cc3ba77eccf.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/bim-antibody-bcl-2-like-protein-11-bha17136091","provider":"BioHippo","version":"1.0","type":"link"}