{"product_id":"bin1-antibody-bridging-integrator-1-bha17135286","title":"BIN1 Antibody \/ Bridging integrator 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBIN1 Antibody \/ Bridging integrator 1 is a anti-BIN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30B93 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BIN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30B93, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBIN1 antibody recognizes bridging integrator 1, a member of the BAR domain protein family that regulates membrane curvature, endocytosis, and cytoskeletal organization. BIN1 is expressed in multiple tissues, with particularly high levels in skeletal and cardiac muscle as well as the nervous system. BIN1 plays important roles in T tubule biogenesis, clathrin mediated endocytosis, and regulation of actin dynamics. Through these functions, BIN1 contributes to muscle physiology, synaptic vesicle recycling, and cellular trafficking.\u003cbr\u003e\u003cbr\u003eBIN1 antibody is highly relevant for research into neuromuscular disorders, cancer, and Alzheimer disease. Mutations in BIN1 are associated with centronuclear myopathy, a congenital muscle disorder characterized by abnormal nuclei positioning and impaired muscle function. Genome wide association studies have also linked BIN1 to increased risk of Alzheimer disease, suggesting that altered BIN1 expression or function contributes to neurodegeneration. In oncology, BIN1 has been identified as a tumor suppressor, where reduced expression correlates with enhanced proliferation and invasion.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. BIN1 antibody detects protein bands corresponding to different isoforms, which arise from extensive alternative splicing. In immunohistochemistry, the antibody highlights tissue specific localization, including strong signals in muscle fibers and neuronal tissues. Immunofluorescence enables detailed visualization of BIN1 distribution along membranes and T tubules, reflecting its role in membrane remodeling.\u003cbr\u003e\u003cbr\u003eBIN1 is also a key regulator of signaling pathways. It interacts with c Myc and modulates transcriptional activity, contributing to its tumor suppressor function. By detecting BIN1 expression with specific antibodies, researchers can explore how changes in BIN1 contribute to malignant transformation. In neuroscience, altered BIN1 expression is thought to affect tau pathology and amyloid processing, making it an important candidate in Alzheimer disease research.\u003cbr\u003e\u003cbr\u003eBIN1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BIN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BIN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BIN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BIN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BIN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047289184621,"sku":"FY12383","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6ca6bcc1-d816-4529-ad4f-cdf31f19f97d.jpg?v=1772019289","url":"https:\/\/www.ebiohippo.com\/products\/bin1-antibody-bridging-integrator-1-bha17135286","provider":"BioHippo","version":"1.0","type":"link"}