{"product_id":"blastaq-2x-qpcr-mastermix-bht10900015","title":"BlasTaq™ 2X qPCR MasterMix-500RXN","description":"\u003cp\u003e\u003cstrong\u003eBlasTaq™ 2X qPCR MasterMix\u003c\/strong\u003e provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains \u003cstrong\u003eBlasTaq™ DNA Polymerase\u003c\/strong\u003e, a next-generation Taq polymerase strategically engineered by \u003cstrong\u003eabm\u003c\/strong\u003e to deliver faster extension rates and stronger overall performance.\u003c\/p\u003e\n\u003cp\u003eWith optimized reaction conditions, \u003cstrong\u003eBlasTaq™ DNA Polymerase\u003c\/strong\u003e offers significantly \u003cstrong\u003eimproved processivity, higher yields, and enhanced sensitivity\u003c\/strong\u003e. These improvements allow for \u003cstrong\u003efaster reaction times\u003c\/strong\u003e, shortening qPCR runs by \u003cstrong\u003eup to 70%\u003c\/strong\u003e compared to reactions using wild-type Taq polymerase. BlasTaq™ possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but lacks 3’-5’ exonuclease activity. It also produces 3’-dA-tailed amplicons, making qPCR products generated with BlasTaq™ compatible with TA cloning systems.\u003c\/p\u003e\n\u003cp\u003eThe MasterMix contains a \u003cstrong\u003efluorescent dye\u003c\/strong\u003e compatible with commonly used detection systems, such as \u003cstrong\u003eSYBR Green™\u003c\/strong\u003e and \u003cstrong\u003eEvaGreen™\u003c\/strong\u003e. Additionally, \u003cstrong\u003eROX Reference Dye\u003c\/strong\u003e is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the \u003ca\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/www.abmgood.com\/uploads\/document\/ROX%20Machine%20Compatibility%20List_v1.pdf\" target=\"_blank\"\u003eROX Machine Compatibility Chart\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eEnable streamlined protocol in a simple reaction set-up\u003c\/li\u003e\n\u003cli\u003eAllow accurate quantification of a variety of gene targets\u003c\/li\u003e\n\u003cli\u003eReduce pipetting steps to minimize the risk of contamination\u003c\/li\u003e\n\u003cli\u003eCompatible with most real-time PCR instruments\u003c\/li\u003e\n\u003cli\u003eContains dye comparable to SYBR Green™ and EvaGreen™ \u003cbr\u003e(Probe-based qPCR Master Mix also available)\u003c\/li\u003e\n\u003cli\u003eIncluded in \u003cstrong\u003eabm\u003c\/strong\u003e's \u003ca\u003ePCR Buffet Program\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFor a larger size, see \u003ca\u003eCat. No. G892\u003c\/a\u003e (2,500 rxn, 25.0 ml)\u003c\/p\u003e\n\u003cp style=\"margin: 20px 0;\"\u003e \u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin-bottom: 20px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Component\u003c\/th\u003e\n\u003cth\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003eBlasTaq™ 2X qPCR MasterMix\u003c\/td\u003e\n\u003ctd\u003e500 rxn (4 x 1.25 ml)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eROX Reference Dye\u003c\/td\u003e\n\u003ctd\u003e50 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003ctable style=\"border-collapse: collapse; background-color: #d6effa; border-radius: 10px; vertical-align: middle; margin-bottom: 20px;\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr valign=\"middle\"\u003e\n\u003ctd style=\"padding: 30px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eCustomer Testimonial\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\"Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols.\"\u003c\/p\u003e\n\u003cp\u003e- Dr. Vivek Vaish, Pune University\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\" border=\"0\" cellpadding=\"10\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"vertical-align: middle;\"\u003e\n\u003ctd style=\"text-align: left; vertical-align: middle;\" width=\"15%\"\u003e\u003cimg src=\"https:\/\/www.abmgood.com\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\" alt=\"ISO 13485:2016 MDSAP Certified\" width=\"85%\"\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\"\u003e\n\u003cp\u003e\u003cstrong\u003eISO 13485:2016 MDSAP Certified\u003c\/strong\u003e\u003cbr\u003eOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"abm-specifications-concentration\"\u003e\n\u003ctd class=\"abm-specifications-label\" valign=\"top\"\u003eConcentration\u003c\/td\u003e\n\u003ctd class=\"abm-specifications-value\" valign=\"top\"\u003e2X\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"abm-specifications-storage_condition\"\u003e\n\u003ctd class=\"abm-specifications-label\" valign=\"top\"\u003eStorage Condition\u003c\/td\u003e\n\u003ctd class=\"abm-specifications-value\" valign=\"top\"\u003e\n\u003cp\u003eStore at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"abm-specifications-note\"\u003e\n\u003ctd class=\"abm-specifications-label\" valign=\"top\"\u003eNote\u003c\/td\u003e\n\u003ctd class=\"abm-specifications-value\" valign=\"top\"\u003e\n\u003cp\u003eBlasTaq™ 2X qPCR MasterMix includes a separate vial of ROX Reference Dye. You can add this dye if your specific qPCR machine requires ROX, as indicated in the table below:\u003c\/p\u003e\n\u003cdiv\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"white-space: nowrap;\"\u003eMachine Type\u003c\/th\u003e\n\u003cth\u003eMachine\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"white-space: nowrap;\"\u003eHigh ROX Machines\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003eABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series\u003c\/li\u003e\n\u003cli\u003eBiometra TOptical\u003c\/li\u003e\n\u003cli\u003eFluidigm BioMark™\u003c\/li\u003e\n\u003cli\u003eWafergene SmartChip System\u003c\/li\u003e\n\u003cli\u003eTianLong TL998 System\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"white-space: nowrap;\"\u003eLow ROX Machines\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003eABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3\/5\/6\/7\u003c\/li\u003e\n\u003cli\u003eBioGene InSyte™\u003c\/li\u003e\n\u003cli\u003eIllumina Eco\u003c\/li\u003e\n\u003cli\u003eAnalytikjena qTower Series\u003c\/li\u003e\n\u003cli\u003eStratagene® Mx3000, Mx3005, Mx4000\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"white-space: nowrap;\"\u003eNo ROX Machines\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003eBioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™\u003c\/li\u003e\n\u003cli\u003eRoche LightCycler® (480, 1536, Nano)\u003c\/li\u003e\n\u003cli\u003eMJ Research Opticon™, Opticon™ 2, Chromo® 4\u003c\/li\u003e\n\u003cli\u003eEppendorf™ Realplex 4\u003c\/li\u003e\n\u003cli\u003eBioGene SynChron™\u003c\/li\u003e\n\u003cli\u003eCorbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)\u003c\/li\u003e\n\u003cli\u003eEppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)\u003c\/li\u003e\n\u003cli\u003eCepheid SmartCyler, GeneXpert\u003c\/li\u003e\n\u003cli\u003eEnigma™ ML\u003c\/li\u003e\n\u003cli\u003eIdaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS\u003c\/li\u003e\n\u003cli\u003eQiagen Rotor-Gene™ (Q, 6000)\u003c\/li\u003e\n\u003cli\u003eTakara Dice™\u003c\/li\u003e\n\u003cli\u003eThermo Scientific PikoReal\u003c\/li\u003e\n\u003cli\u003eDNA-Technology DT96, DTlite, DT-322\u003c\/li\u003e\n\u003cli\u003eBioer LineGene (3310\/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)\u003c\/li\u003e\n\u003cli\u003eBioneer Exicycler™\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"500 rxn (4 x 1.25 ml)","offer_id":53311770001773,"sku":"G891","price":139.0,"currency_code":"USD","in_stock":true},{"title":"125 rxns (1.25ml)","offer_id":53311770952045,"sku":"Sample-G891","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/g891-2-blastaq-2x-qpcr-mastermix_product-image.jpg?v=1778555686","url":"https:\/\/www.ebiohippo.com\/products\/blastaq-2x-qpcr-mastermix-bht10900015","provider":"BioHippo","version":"1.0","type":"link"}