{"product_id":"blood-cell-tissue-genomic-dna-extraction-kit-bht15200008","title":"Blood\/Cell\/Tissue Genomic DNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe kit is suitable for extracting high purity total DNA from fresh or frozen animal tissues, cells, blood, bacteria and other samples. DNA fragments up to 50 kb in length can be purified without the use of toxic solvents such as phenol or chloroform and ethanol precipitation. An optimized buffer system binds DNA efficiently and specifically from the lysate to the silica-based spin column. PCR and other inhibitors of enzymatic reaction could be effectively removed by two-step washing steps. Finally, high purity DNA could be obtained by using low-salt buffer or water elution. The purified DNA can be directly used in enzyme digestion, PCR, Real-Time PCR, library construction, Southern Blot, molecular markers and other downstream experiments.\u003c\/p\u003e\n\u003cp\u003eThe sample is digested with 20 µL of Proteinase K and 200 µL of Solution GS at 56°C for 10 min, then mixed with 350 µL of Solution GA2 and 350 µL of absolute ethanol and loaded onto the adsorption column. The column is washed with 500 µL of Solution RP and 600 µL of Wash Buffer, dried by centrifugation at 12,000 rpm for 2 min, and eluted with 50–200 µL of Solution GE or sterilized water after 2–5 min at room temperature. Purified DNA has an OD260\/OD280 of 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica-based spin column (Adsorption column G)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eFresh or frozen animal tissue, cells, blood and bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, blood\u003c\/th\u003e\n\u003ctd\u003e200 µL standard, 300 µL–1 mL with additional processing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, bacteria\u003c\/th\u003e\n\u003ctd\u003e1–30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMaximum fragment size\u003c\/th\u003e\n\u003ctd\u003e50 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003ePhenol\/chloroform-free; no ethanol precipitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eOD260\/OD280 should be 1.7–1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e50–200 µL of Solution GE or water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution GA1\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution GS\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution GA2\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003ctd\u003e120 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution RP\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution GE\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column G column\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003ctd\u003e200 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenomic DNA extraction from tissue, cells, blood and bacteria\u003c\/li\u003e\n\u003cli\u003eEnzyme digestion, PCR and Real-Time PCR\u003c\/li\u003e\n\u003cli\u003eLibrary construction\u003c\/li\u003e\n\u003cli\u003eSouthern Blot and molecular marker analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRapid purification yields high-quality, ready-to-use DNA.\u003c\/li\u003e\n\u003cli\u003eGood repeatability and high output.\u003c\/li\u003e\n\u003cli\u003eEffectively removes contaminants and inhibitors, simplifying downstream applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eProteinase K is stored at -20°C for 1 year. Solutions GA1, GS, GA2, RP and GE, the Wash Buffer and the adsorption columns are stored at room temperature for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freezing and thawing of samples.\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the Wash Buffer and Solution RP before use.\u003c\/li\u003e\n\u003cli\u003eCheck the solutions for crystallization and dissolve them at 37°C if needed.\u003c\/li\u003e\n\u003cli\u003eConfirm that digestion has produced a clear solution before loading the column.\u003c\/li\u003e\n\u003cli\u003eRemove residual ethanol from the column before elution.\u003c\/li\u003e\n\u003cli\u003eIf eluting with water instead of Solution GE, use water at pH 7.0–8.5.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP007\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200008\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965511819629,"sku":"EP007-50T","price":130.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965516341613,"sku":"EP007-200T","price":260.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965516374381,"sku":"EP007-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965516407149,"sku":"EP007-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP007-a-1.jpg?v=1789419186","url":"https:\/\/www.ebiohippo.com\/products\/blood-cell-tissue-genomic-dna-extraction-kit-bht15200008","provider":"BioHippo","version":"1.0","type":"link"}