{"product_id":"bop1-antibody-block-of-proliferation-1-bha17135452","title":"BOP1 Antibody \/ Block of proliferation 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBOP1 Antibody \/ Block of proliferation 1 is a anti-BOP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BOP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBOP1 antibody detects Block of proliferation 1 protein, a nucleolar factor that plays a central role in ribosome biogenesis, cell cycle regulation, and tumor progression. BOP1 forms part of the PeBoW complex (with PES1 and WDR12), which is required for maturation of the 28S and 5.8S ribosomal RNAs and assembly of the 60S ribosomal subunit. The BOP1 antibody is used in studies of ribosome formation, cellular proliferation, and oncogenic transformation.\u003cbr\u003e\u003cbr\u003eBOP1 is encoded by the BOP1 gene located on human chromosome 8q24.3, a genomic region frequently amplified in cancers. The protein is approximately 80 kilodaltons and contains WD40 repeat motifs that mediate protein-protein interactions within the nucleolus. BOP1 functions as a structural scaffold coordinating pre-rRNA processing and ribonucleoprotein complex formation. Its proper activity is critical for balanced ribosome production and maintenance of genomic stability.\u003cbr\u003e\u003cbr\u003eThe BOP1 antibody detects a 100-110 kilodalton band by western blot and exhibits strong nucleolar localization by immunofluorescence. During ribosome assembly, BOP1 interacts with PES1 and WDR12 to stabilize preribosomal particles, ensuring efficient cleavage and processing of rRNA precursors. Loss of BOP1 results in accumulation of immature rRNA intermediates and nucleolar stress, leading to p53 activation and cell cycle arrest.\u003cbr\u003e\u003cbr\u003eBOP1 also influences mitotic progression and centrosome duplication. Overexpression accelerates cell proliferation and promotes chromosomal instability, while depletion inhibits tumor cell growth. Dysregulation of BOP1 has been reported in colorectal, pancreatic, and hepatocellular carcinomas, correlating with poor prognosis and increased metastatic potential.\u003cbr\u003e\u003cbr\u003eBecause ribosome biogenesis is tightly linked to cell growth, BOP1 serves as a valuable biomarker for cancer diagnostics and as a potential target for anti-proliferative therapies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BOP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BOP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BOP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BOP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BOP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293509997,"sku":"FY12549","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f11f2710-f141-437e-adca-673341da44c2.jpg?v=1782237031","url":"https:\/\/www.ebiohippo.com\/products\/bop1-antibody-block-of-proliferation-1-bha17135452","provider":"BioHippo","version":"1.0","type":"link"}