{"product_id":"bovine-ap-aprotinin-competitive-elisa-kit-ready-to-use-kit-stje0013569-bhe15511384","title":"Bovine AP (Aprotinin) Competitive ELISA Kit Ready-To-Use Kit (STJE0013569)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:22px}\n.bhp-desc li{margin-bottom:4px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin:4px 6px 10px 0}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px;display:inline-block;margin:3px 4px 3px 0}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin:0 6px 10px 0}\n\u003c\/style\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAP (Aprotinin)\u003c\/strong\u003e is a biomarker protein expressed in bovine tissues and biological fluids. Dysregulation of Aprotinin has been implicated in cellular signaling and fundamental cell biology, making it a relevant biomarker for investigational studies.\u003c\/p\u003e\n\u003cp\u003eQuantitative measurement of Aprotinin enables researchers to characterize its abundance in Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant and correlate levels with experimental conditions. Studies investigating Aprotinin have contributed to the understanding of cell signaling pathways, disease pathogenesis, and therapeutic target validation.\u003c\/p\u003e\n\u003cp\u003eThis competitive ELISA kit provides a validated, reproducible format for Aprotinin quantification within the inhibition range of 1.56-100ng\/mL. The assay supports experimental workflows in Cell Biology research.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eEach kit contains sufficient reagents for one 96-well plate (approximately 40 samples in duplicate with standard curve):\u003c\/p\u003e\n\u003cp\u003ePre-coated microplate (96 wells), HRP-conjugated antigen, standard protein, sample diluent, wash buffer, TMB substrate solution, stop solution, and product manual.\u003c\/p\u003e\n\u003cspan class=\"bhp-qc-badge\"\u003eValidated Lot\u003c\/span\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eCompetitive ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56-100ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity (LLOD)\u003c\/th\u003e\n\u003ctd\u003e0.43ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidated Sample Types\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e2.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (450 nm \/ 540–570 nm reference)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e96-well microplate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eBovine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt Accession\u003c\/th\u003e\n\u003ctd\u003eP00974\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNCBI Gene ID\u003c\/th\u003e\n\u003ctd\u003e404172\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eThe whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure Summary\u003c\/h2\u003e\n\u003cp\u003eAdd samples and HRP-conjugated antigen simultaneously to pre-coated wells and incubate. Wash, add TMB substrate, and read at 450 nm. Signal is inversely proportional to AP concentration. Construct a standard inhibition curve from the provided reference antigen to calculate sample concentrations. Total protocol time: approximately 2.5 hours.\u003c\/p\u003e\n\u003cp\u003ePre-warm all kit components to room temperature (18–25 °C) for 30 minutes before starting. Run all samples in duplicate. Centrifuge samples at ≥1,000 × g before use to remove particulates.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Intended Use\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle in accordance with good laboratory practice.\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eIntended Use\u003c\/strong\u003eResearch Use Only (RUO)\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eVendor\u003c\/strong\u003eSt John's Laboratory Ltd\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003eSTJE0013569\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003eBovine\u003c\/span\u003e\u003cspan class=\"bhp-reg-item\"\u003e\u003cstrong\u003eUniProt\u003c\/strong\u003eP00974\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"48 T","offer_id":53335888101741,"sku":"STJE0013569-48","price":649.29,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53335889903981,"sku":"STJE0013569-96","price":905.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/STJE0007193_1__19115.1682604840.png?v=1778912833","url":"https:\/\/www.ebiohippo.com\/products\/bovine-ap-aprotinin-competitive-elisa-kit-ready-to-use-kit-stje0013569-bhe15511384","provider":"BioHippo","version":"1.0","type":"link"}