{"product_id":"braf-v600e-antibody-bha17133040","title":"BRAF V600E Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBRAF V600E Antibody is an antibody targeting \u003cstrong\u003eBRAF\u003c\/strong\u003e. Host: \u003cstrong\u003eMouse\u003c\/strong\u003e. Monoclonal. Clone: \u003cstrong\u003eV600E\/1321\u003c\/strong\u003e. Format: Purified. Applications: ELISA.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BRAF.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e An N-terminal KLH-conjugated peptide (amino acids 596-606, Cys-GLAT(E)KSRWSG) was used as the immunogen for the BRAF V6….\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasm.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated (no label listed).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background (from provided description)\u003c\/h2\u003e\u003cp\u003eThe BRAF gene encodes a cytoplasmic serine-threonine kinase, which initiates the activation of the mitogen-activated protein kinase (MAPK) signalling pathway. The oncogenic mutations in the kinase region of BRAF gene result in constitutive activation of the MAPK signalling pathway, leading to increased cell proliferation, resistance to apoptosis and tumor progression.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eSuitable for antibody-based detection workflows where target signal comparison and localization are required.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAccount for isoforms, paralogs, and post-translational modifications when interpreting signal.\u003c\/li\u003e\n\u003cli\u003eSupport specificity with biological controls (e.g., KO\/KD where available) and technical controls (e.g., secondary-only\/isotype).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt) – https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NLM) – https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - ZFIN – https:\/\/zfin.org\/ - Ensembl – https:\/\/www.ensembl.org\/ - Nature Methods (2015): A proposal for validation of antibodies – https:\/\/www.nature.com\/articles\/nmeth.3230 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 100 ug","offer_id":53046679699821,"sku":"V5229-100UG","price":559.0,"currency_code":"USD","in_stock":true},{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 20 ug","offer_id":53046823649645,"sku":"V5229-20UG","price":259.0,"currency_code":"USD","in_stock":true},{"title":"1 mg\/ml in 1X PBS; BSA free, sodium azide free \/ 100 ug","offer_id":53046823682413,"sku":"V5229SAF-100UG","price":559.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c39e52a8-729e-4f47-8ab3-94c17dfa1be0.jpg?v=1772010168","url":"https:\/\/www.ebiohippo.com\/products\/braf-v600e-antibody-bha17133040","provider":"BioHippo","version":"1.0","type":"link"}