{"product_id":"brdu-cell-proliferation-colorimetric-assay-kit-bht19600043","title":"BrdU Cell Proliferation Colorimetric Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eCell Proliferation Assay Kit\u003c\/span\u003e\u003ch2\u003eReadout \u0026amp; Interpretation\u003c\/h2\u003e\n\u003cp\u003eBromodeoxyuridine is a thymidine analogue that is incorporated into newly synthesised DNA only in cells that are actively replicating, allowing proliferation to be quantified without counting the cells that were merely seeded.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe BrdU Cell Proliferation Colorimetric Assay Kit applies a colorimetric format to this measurement, read at 370 nm (ref 492 nm) without stop solution; 450 nm (ref 690 nm) with stop solution.\u003c\/p\u003e\n\u003cp\u003eThe extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eColorimetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eAbsorbance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003e370 nm (ref 492 nm) without stop solution; 450 nm (ref 690 nm) with stop solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003eas few as 50-100 proliferating cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Types\u003c\/th\u003e\n\u003ctd\u003eadherent cells, suspension cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e200 tests\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore 1000x BrdU Labeling Reagent and 100x Anti-BrdU-POD at -20°C; Antibody Diluent, 10x Washing Buffer, FixDenat, TMB Substrate and Stop Solution at 4°C. Shelf-life: 12 months after receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell Proliferation\u003c\/li\u003e\n\u003cli\u003eCytotoxicity Assay\u003c\/li\u003e\n\u003cli\u003eCompound Profiling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStore 1000x BrdU Labeling Reagent and 100x Anti-BrdU-POD at -20°C; Antibody Diluent, 10x Washing Buffer, FixDenat, TMB Substrate and Stop Solution at 4°C. Shelf-life: 12 months after receipt.\u003c\/p\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eComponents of this kit are stored at two different temperatures. Unpack on arrival and file each component at the temperature stated above.\u003c\/div\u003e\n\u003ch2\u003eSafety\u003c\/h2\u003e\n\u003cp\u003eFor research use only. Not for use in diagnostic or therapeutic procedures. Handle in accordance with good laboratory practice; consult the SDS before use.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Tribioscience","offers":[{"title":"200 tests","offer_id":53652623262061,"sku":"TBS2086","price":456.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TBS2086.jpg?v=1785879139","url":"https:\/\/www.ebiohippo.com\/products\/brdu-cell-proliferation-colorimetric-assay-kit-bht19600043","provider":"BioHippo","version":"1.0","type":"link"}