{"product_id":"btaf1-antibody-hrp-conjugated-bha10529005","title":"BTAF1 Antibody, HRP conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-BTAF1 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect BTAF1 in Human for epigenetics and nuclear signaling research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human TATA-binding protein-associated factor 172 protein (1750-1849AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHRP:\u003c\/strong\u003e Direct detection (TMB\/ECL); quench endogenous peroxidase in tissue assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eBTAF1\u003c\/strong\u003e (also: ATP dependent helicase BTAF1, ATP-dependent helicase BTAF1, B-TFIID transcription factor associated 170 kDa subunit, B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, BTAF 1 RNA polymerase II, BTAF1, BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa, BTAF1_HUMAN, MOT 1, MOT1, MOT1 Yeast homolog of, TAF 172, TAF II 170, TAF(II)170, TAF-172, TAF172, TAFII170, TATA binding protein associated factor 172, TATA-binding protein-associated factor 172, TBP associated factor 172, TBP-associated factor 172) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current BTAF1 annotation relevant to epigenetics and nuclear signaling.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240844452205,"sku":"CSB-PA002839LB01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240947278189,"sku":"CSB-PA002839LB01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_59ee1bc1-1408-49a9-b3d6-041d16931a1d.jpg?v=1784926927","url":"https:\/\/www.ebiohippo.com\/products\/btaf1-antibody-hrp-conjugated-bha10529005","provider":"BioHippo","version":"1.0","type":"link"}