{"product_id":"c-ebp-beta-reporter-lentivirus-bhv19400029","title":"C\/EBP-β Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eC\/EBP-β (CCAAT\/enhancer-binding protein beta), encoded by CEBPB, is a bZIP transcription factor that binds CCAAT-box DNA elements to regulate gene expression. It is a key regulator of adipocyte differentiation, where it acts early in the transcriptional cascade that activates PPARγ and drives terminal adipogenesis. C\/EBP-β also controls the acute-phase inflammatory response, macrophage function, and metabolic gene expression in liver and other tissues. Through its roles in metabolism, immunity, and cell differentiation, C\/EBP-β is widely studied in metabolic disease, inflammation, and cell signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe C\/EBP-β Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem consensus C\/EBP DNA-binding elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of C\/EBP-β transcriptional activity in transduced cells. Reporter options include GFP, RFP, and luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring C\/EBP pathway activation, studying adipocyte differentiation and inflammatory signaling, and screening compounds that modulate C\/EBP-β activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a GFP, Luc, RFP reporter gene under the control of tandem consensus response elements specific for the C\/EBP-b, PPAR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616211309,"sku":"LTV-0033-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310898602349,"sku":"LTV-0033-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310898635117,"sku":"LTV-0033-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310898667885,"sku":"LTV-0033-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310898700653,"sku":"LTV-0033-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310898733421,"sku":"LTV-0033-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0033-CEBPB-Reporter-Fig1.jpg?v=1776934149","url":"https:\/\/www.ebiohippo.com\/products\/c-ebp-beta-reporter-lentivirus-bhv19400029","provider":"BioHippo","version":"1.0","type":"link"}