{"product_id":"c3-recombinant-monoclonal-antibody-bha10576380","title":"C3 Recombinant Monoclonal Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a monoclonal anti-C3 antibody raised in Human, with confirmed utility in ELISA, FC. It is designed to detect C3 protein in Human and supports researchers working in others contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human C3 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Human):\u003c\/strong\u003e Requires anti-human-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonoclonal format:\u003c\/strong\u003e Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG1):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Affinity chromatography):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eC3\u003c\/strong\u003e (also referred to as ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3, C3adesArg, C3bc, CO3_HUMAN, Complement C3c alpha'' chain fragment 2) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of C3 biology in others.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFC:\u003c\/strong\u003e Applicable for FC-based detection; refer to product documentation for optimized conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG1 from Human) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Confirm the clone does not cross-react with related family members under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a recombinant monoclonal antibody expressed in HEK293F cells.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"50 uL","offer_id":53248416219501,"sku":"CSB-RA355590MA1HU-50UL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53248489521517,"sku":"CSB-RA355590MA1HU-100UL","price":350.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/c3-recombinant-monoclonal-antibody-bha10576380","provider":"BioHippo","version":"1.0","type":"link"}