{"product_id":"calfectin-mammalian-cell-transfection-reagent-bht2200002","title":"CalFectin™ Mammalian Cell Transfection Reagent","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-figure{margin:16px 0 20px;text-align:center}.bhp-desc .bhp-figure img{max-width:100%;height:auto;display:block;margin:0 auto 6px}.bhp-desc .bhp-figure figcaption{font-size:14px;color:#444;text-align:left;line-height:1.5}.bhp-desc .bhp-data-table th{width:auto}.bhp-desc .bhp-data-table td{font-size:14px}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eCalFectin™ Mammalian DNA Transfection Reagent is a refined, pre-optimized calcium phosphate–based transfection reagent built on a novel chemistry. It carries a significantly lower calcium concentration than traditional calcium phosphate methods, which markedly reduces cytotoxicity while transfection efficiency is not compromised. The reagent works highly effectively for both transient and long-term stable transfection across most adherent mammalian cell lines.\u003c\/p\u003e\n\u003cp\u003eAccording to SignaGen, CalFectin™ outperforms liposome- and polymer-based transfection reagents, with superior performance and higher efficiency in a wide range of cell types, among them HepG2, HEK293, CHO, HeLa, COS, NIH\/3T3, LNCaP, C6, PC12, and primary cultured cells. A single 1.0 mL vial of CalFectin™ Reagent covers roughly 666 transfections in 24-well plates or 333 transfections in 6-well plates.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTransfection Type\u003c\/th\u003e\n\u003ctd\u003eDNA Transfection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication Setting\u003c\/th\u003e\n\u003ctd\u003eIn Vitro\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePre-Optimized\u003c\/th\u003e\n\u003ctd\u003eNo — General-Purpose Formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4 °C. If stored properly, the product is stable for 12 months or longer.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh efficiency: components optimized for high transfection efficiencies\u003c\/li\u003e\n\u003cli\u003eSimple procedure: complete reaction in one tube\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectvsL2K-1.jpg?v=1790173423\" alt=\"CalFectin™ Mammalian Cell Transfection Reagent – GFP-tagged 14 kb DNA transfection in HEK293T cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison study showing transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HEK293T cells. HEK293T cells were transfected with GFP-tagged DNA (total 14 kb) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003cfigure class=\"bhp-figure\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectvsHD-3.jpg?v=1790173423\" alt=\"CalFectin™ Mammalian Cell Transfection Reagent – GFP transfection in HepG2 cells vs Lipofectamine 2000\" loading=\"lazy\"\u003e\u003cfigcaption\u003eA comparison study showing the transfection efficiency of CalFectin™ Reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. HepG2 cells were transfected with GFP vector (pEGFP-N3) by CalFectin™ (left panel) and Lipofectamine 2000 (right panel), respectively. The cells were visualized by a Nikon Eclipse Fluorescence microscope 24 hours post-transfection\u003c\/figcaption\u003e\u003c\/figure\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eStable or transient transfection of eukaryotic cells at high efficiency\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e — Not for diagnostic or therapeutic use. Handle reagents according to institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"0.1 mL","offer_id":54042898858349,"sku":"SL100478-0P1ML","price":20.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mL","offer_id":54042901315949,"sku":"SL100478-0P5ML","price":68.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL","offer_id":54042901348717,"sku":"SL100478-1P0ML","price":118.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mL x 5","offer_id":54042901381485,"sku":"SL100478-1P0MLX5","price":560.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/signagen-CalFectin-vs-L2K_jpg_6e01a95f-0d8c-40a2-984e-757609d4e24b.webp?v=1790175945","url":"https:\/\/www.ebiohippo.com\/products\/calfectin-mammalian-cell-transfection-reagent-bht2200002","provider":"BioHippo","version":"1.0","type":"link"}