{"product_id":"canine-lymph-node-metastasis-tcc-cell-line-ku-ctcc-001-lm3-bhc10902050","title":"Canine Lymph Node Metastasis TCC Cell Line (KU-CTCC-001-LM3)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eKU-CTCC-001-LM3, established from a metastatic lymph node of the same patient as KU-CTCC-001 (Cat. T8165), harbors the BRAF V595E mutation and displays activated MAPK signaling.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Canine Lymph Node Metastasis TCC Cell Line (KU-CTCC-001-LM3) is supplied as a tumor cell line derived from Dog lymph node.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, round to spindle\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow XXII Medium (TM122) + 10% FBS(Regular*) + 10 mM HEPES (TM058) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in lymph node biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis cell line presents a unique model to study metastatic processes, offering insights into the molecular and cellular mechanisms underlying TCC spread. Its growth characteristics and sensitivity to BRAF inhibitors, including a notable response to sorafenib, make it a critical tool for advancing understanding of metastasis, evaluating therapeutic strategies for metastatic disease, and contributing to the development of personalized medicine approaches in canine and human oncology.Note: KU-CTCC-001-LM3, derived from a metastatic site, enables focused research on the metastatic behavior and potential therapeutic targets specific to TCC metastasis, compared to KU-CTCC-001 (Cat.T8165) and KU-CTCC-002 (Cat.T8166), which are derived from primary tumors. Its slightly slower proliferation rate in vitro versus KU-CTCC-001 (Cat. T8165) might reflect differences in growth dynamics between primary and metastatic cancer cells.Complete list of cell lines from this panel:Cat. T8165 - Canine Transitional Cell Carcinoma Cell Line (KU-CTCC-001)Cat. T8166 - Canine Transitional Cell Carcinoma Cell Line (KU-CTCC-002) Cat. T8167 - Canine Lymph Node Metastasis TCC Cell Line (KU-CTCC-001-LM3) Donor\/background information provided for this product: 10, Jindo, Transitional Cell Carcinoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow XXII Medium (TM122) + 10% FBS(Regular*) + 10 mM HEPES (TM058) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.*Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 40,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180539732333,"sku":"T8167","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VJveRKPee2lTxwxtVqlIJRz4bPiO9UUhe6MgzscH.png?v=1774957852","url":"https:\/\/www.ebiohippo.com\/products\/canine-lymph-node-metastasis-tcc-cell-line-ku-ctcc-001-lm3-bhc10902050","provider":"BioHippo","version":"1.0","type":"link"}