{"product_id":"canine-primary-bone-marrow-monocytes-bhc10923196","title":"Canine Primary Bone Marrow Monocytes","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCanine Primary Bone Marrow Monocytes are dog frozen primary cells from bone marrow used for innate immune signaling, phagocytic response, and inflammatory pathway studies. These cells display suspension, round growth characteristics.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Dog (Canine)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Bone Marrow\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, round\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Leukocyte Medium Kit (TM164) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eMonocyte- and macrophage-lineage cells coordinate innate immune sensing, cytokine production, phagocytosis, and tissue-specific inflammatory responses.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary innate immune cell models are widely used to assess activation-state plasticity and cytokine output.\u003c\/li\u003e\n\u003cli\u003eResearchers often study tissue-specific macrophage phenotypes or donor-dependent inflammatory responses.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems help reveal crosstalk with stromal, epithelial, endothelial, or parenchymal cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure inflammatory mediator release and activation markers after stimulation.\u003c\/li\u003e\n\u003cli\u003eUse in phagocytosis-focused or co-culture assays requiring primary innate immune cells.\u003c\/li\u003e\n\u003cli\u003eEvaluate tissue-specific immune crosstalk in organ-relevant model systems.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Leukocyte Medium Kit (TM164) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10923196\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195757977965,"sku":"T4157","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FL1zgDpE8H66ZYxmW2Y2K7tDmOTeYNw8fjcJALq7.png?v=1775322490","url":"https:\/\/www.ebiohippo.com\/products\/canine-primary-bone-marrow-monocytes-bhc10923196","provider":"BioHippo","version":"1.0","type":"link"}