{"product_id":"canine-primary-dermal-fibroblasts-bhc10900020","title":"Canine Primary Dermal Fibroblasts","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCanine Primary Dermal Fibroblasts are isolated from healthy dog skin tissue and exhibit typical spindle-shaped fibroblast morphology with robust proliferative capacity. These cells provide a physiologically relevant in vitro model for studying skin biology, extracellular matrix production, wound healing, fibrosis, and dermatological disease mechanisms. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Dog (Canine)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Skin\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, bipolar\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDonor history:\u003c\/strong\u003e Normal tissue\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFibroblasts are stromal cells that synthesize extracellular matrix, respond to mechanical and inflammatory cues, and shape tissue repair programs through paracrine signaling and matrix remodeling.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary fibroblasts are used to study donor-specific matrix production and activation-state changes in vitro.\u003c\/li\u003e\n\u003cli\u003eResearchers increasingly examine fibroblast heterogeneity across tissues and disease states.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems are commonly used to evaluate stromal effects on epithelial, immune, or tumor-associated phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure matrix production, activation markers, and cytokine responses under defined stimuli.\u003c\/li\u003e\n\u003cli\u003eStudy wound-healing, fibrosis-associated, or stromal support phenotypes in tissue-relevant contexts.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture assays to evaluate paracrine effects on neighboring cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Fibroblast Cell Growth Medium Kit (TM107) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells can be expanded for 3-5 passages using a 1:2 split ratio, using the recommended culture conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10900020\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195743199597,"sku":"T0269","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/T0269_Morphology.png?v=1775322472","url":"https:\/\/www.ebiohippo.com\/products\/canine-primary-dermal-fibroblasts-bhc10900020","provider":"BioHippo","version":"1.0","type":"link"}