{"product_id":"capn3-antibody-calpain-3-bha17128195","title":"CAPN3 Antibody \/ Calpain 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eCAPN3 Antibody \/ Calpain 3 is an antibody targeting \u003cstrong\u003eCAPN3\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CAPN3.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); Rabbit Ig.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eCalpain 3 is a member of the calpain family of proteases, which are enzymes that break down proteins in the body. Unlike other calpain enzymes that are present in various tissues, Calpain 3 is predominantly found in skeletal muscle. Its main function is to regulate the turnover of muscle proteins, ensuring that they are properly maintained and repaired. One of the key roles of Calpain 3 is to maintain the structural integrity of muscle fibers. It is involved in the breakdown of damaged or misfolded proteins, preventing them from accumulating and causing muscle dysfunction. Additionally, Calpain 3 plays a vital role in muscle regeneration, helping to repair damaged fibers and promote muscle growth. Deficiencies in Calpain 3 have been linked to various muscle disorders, including limb-girdle muscular dystrophy type 2A. In individuals with this condition, mutations in the CAPN3 gene lead to impaired muscle function and weakness.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eImmunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eFlow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry P20807 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P20807 - Gene search: CAPN3 — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CAPN3 - Ensembl search: CAPN3 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CAPN3 - PubMed search: CAPN3 antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CAPN3+antibody - Reactome search: CAPN3 — Reactome — https:\/\/reactome.org\/content\/query?q=CAPN3 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.08 ml","offer_id":53046409953645,"sku":"F55132-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.4 ml","offer_id":53046471262573,"sku":"F55132-0.4ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b409d8f7-8a42-4b99-a2fd-6e2d0336bacd.jpg?v=1772000497","url":"https:\/\/www.ebiohippo.com\/products\/capn3-antibody-calpain-3-bha17128195","provider":"BioHippo","version":"1.0","type":"link"}