{"product_id":"capn3-antibody-calpain-3-bha17136035","title":"CAPN3 Antibody \/ Calpain 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCAPN3 Antibody \/ Calpain 3 is a anti-CAPN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CAPN3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCAPN3 antibody detects Calpain 3, a skeletal muscle-specific calcium-dependent cysteine protease involved in muscle remodeling and sarcomere maintenance. The UniProt recommended name is Calpain 3 (CAPN3). This enzyme belongs to the calpain family of proteases that regulate cytoskeletal dynamics and muscle protein turnover.\u003cbr\u003e\u003cbr\u003eFunctionally, CAPN3 antibody identifies a 821-amino-acid protease characterized by catalytic cysteine and histidine residues forming the proteolytic triad. Calpain 3 associates with titin in the sarcomere and modulates proteolytic remodeling during muscle contraction and regeneration. Its activity is tightly regulated by calcium binding and autolytic activation mechanisms unique to muscle tissue.\u003cbr\u003e\u003cbr\u003eThe CAPN3 gene is located on chromosome 15q15.1 and is expressed predominantly in skeletal muscle. Calpain 3 acts as a muscle-specific regulator controlling sarcomeric integrity and signaling pathways that respond to mechanical stress and injury. It is rapidly activated upon calcium influx, enabling dynamic structural adaptation.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in CAPN3 cause limb-girdle muscular dystrophy type 2A (LGMD2A), an autosomal recessive disorder characterized by progressive muscle weakness and atrophy. Deficiency of Calpain 3 disrupts sarcomere homeostasis and impairs muscle repair. Research using CAPN3 antibody supports studies in muscle physiology, proteolysis, and dystrophic disease mechanisms.\u003cbr\u003e\u003cbr\u003eCAPN3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect muscle-specific proteases. \u003cbr\u003e\u003cbr\u003eStructurally, Calpain 3 consists of protease core domains (PC1 and PC2) flanked by unique insertion sequences IS1 and IS2, which confer muscle-specific regulation. This antibody aids investigation of CAPN3's enzymatic role in sarcomere remodeling and muscular dystrophy.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CAPN3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CAPN3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CAPN3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CAPN3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CAPN3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313957229,"sku":"FY13133","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c8b56fda-adb2-4d6f-82b6-032f74d69220.jpg?v=1782237100","url":"https:\/\/www.ebiohippo.com\/products\/capn3-antibody-calpain-3-bha17136035","provider":"BioHippo","version":"1.0","type":"link"}