{"product_id":"capn6-antibody-calpain-6-bha17135225","title":"CAPN6 Antibody \/ Calpain 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCAPN6 Antibody \/ Calpain 6 is a anti-CAPN6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, perinuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CAPN6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCAPN6 antibody, also referred to as Calpain 6 antibody, targets a non-proteolytic member of the calpain family of calcium-regulated proteins. Unlike most calpains that act as cysteine proteases, Calpain 6 is catalytically inactive due to divergence in its protease domain. Instead, it exerts regulatory functions that shape cytoskeletal organization, cell migration, and tissue remodeling. CAPN6 is strongly expressed in embryonic and placental tissues, where it supports morphogenesis and vascular development, and it continues to play roles in disease contexts such as cancer and bone biology.\u003cbr\u003e\u003cbr\u003eOne of the primary functions of Calpain 6 is to stabilize microtubules by directly binding to tubulin. Through this interaction, CAPN6 regulates lamellipodia formation and enhances the ability of cells to migrate. This cytoskeletal remodeling is essential for embryonic tissue development, wound healing, and angiogenesis. In endothelial cells, CAPN6 supports vessel formation and vascular integrity, while in osteoclasts it influences bone resorption. Researchers investigating structural dynamics of the cytoskeleton use CAPN6 antibody to trace its expression and monitor its interactions with microtubule networks.\u003cbr\u003e\u003cbr\u003eThe absence of proteolytic activity makes Calpain 6 unique among calpains, highlighting the functional diversity of this protein family. Instead of cleaving substrates, CAPN6 serves as a structural regulator, linking calcium signaling with cytoskeletal architecture. This distinction is particularly important in oncology, where abnormal expression of CAPN6 has been reported in hepatocellular carcinoma, uterine leiomyosarcoma, and endometrial cancer. Overexpression may enhance invasiveness by promoting cytoskeletal plasticity, while downregulation may disrupt normal development and tissue repair. The dual roles of CAPN6 underscore its importance as both a developmental regulator and a potential biomarker of malignancy.\u003cbr\u003e\u003cbr\u003eCAPN6 antibody is versatile in laboratory applications. Western blotting can be used to detect protein levels under conditions that affect cytoskeletal dynamics, such as drug treatments or genetic manipulation. Immunofluorescence allows visualization of CAPN6 co-localization with tubulin, offering insight into how it modulates microtubule stability. Immunohistochemistry with CAPN6 antibody reveals distribution patterns in tumors and placental tissues, enabling researchers to evaluate associations between CAPN6 expression and disease progression. In developmental biology, CAPN6 antibody helps clarify the role of non-proteolytic calpains in tissue morphogenesis and angiogenesis.\u003cbr\u003e\u003cbr\u003eThe CAPN6 antibody provided by \u003cbr\u003e\u003cbr\u003eAs the only calpain family member lacking enzymatic activity, Calpain 6 occupies a unique position in protein biology. Its ability to regulate cell migration and cytoskeletal remodeling without protease function has expanded understanding of how calpains contribute to physiology and disease. By employing CAPN6 antibody, researchers can investigate how this protein influences endothelial biology, osteoclast activity, tumor invasion, and placental function. These insights not only clarify the role of CAPN6 in normal development but also suggest therapeutic relevance in oncology and regenerative medicine. For laboratories studying the intersection of cytoskeletal dynamics and cellular signaling, CAPN6 antibody remains an essential resource, supported by the consistent quality of\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CAPN6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CAPN6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CAPN6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CAPN6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CAPN6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284531565,"sku":"FY12322","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5d43909f-ca2d-468d-9025-0c6f0f6f6540.jpg?v=1782236990","url":"https:\/\/www.ebiohippo.com\/products\/capn6-antibody-calpain-6-bha17135225","provider":"BioHippo","version":"1.0","type":"link"}