{"product_id":"care-reporter-lentivirus-bhv19400222","title":"CARE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCAR (constitutive androstane receptor, NR1I3) is a ligand-activated transcription factor of the nuclear receptor superfamily and a master regulator of xenobiotic and drug metabolism. CAR heterodimerizes with the retinoid X receptor alpha (RXRα) and binds CAR response elements (CARE), often arranged as direct repeats, in the promoters of cytochrome P450 and other detoxification genes. CAR can be activated through both direct ligand binding and ligand-independent mechanisms, and the CAR-RXRα heterodimer participates in drug metabolism, energy homeostasis, and the modulation of cancer development by regulating numerous target genes, predominantly in hepatocytes. These functions make CARE-driven transcription an informative readout in metabolism and pharmacology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CARE Reporter Lentivirus is a transcription-factor reporter system engineered to detect the transcriptional activity of the constitutive androstane receptor in human and mouse cells. The construct uses tandem repeats of CAR response elements derived from the cytochrome P450 (CYP2B10) gene promoter, arranged in a DR4 pattern to read out the synergistic activity of the CAR\/RXRα heterodimer characteristic of hepatocytes. A fluorescent or luminescent reporter is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying CAR-mediated drug metabolism, energy homeostasis, and CAR pharmacology. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the CAR1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616014701,"sku":"LTV-0113-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362028282221,"sku":"LTV-0113-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362028314989,"sku":"LTV-0113-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362028347757,"sku":"LTV-0113-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362028380525,"sku":"LTV-0113-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362028413293,"sku":"LTV-0113-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CARE-TAG-PURO-FIG1.jpg?v=1776934133","url":"https:\/\/www.ebiohippo.com\/products\/care-reporter-lentivirus-bhv19400222","provider":"BioHippo","version":"1.0","type":"link"}