{"product_id":"carf-antibody-collaborator-of-arf-cdkn2aip-bha17136281","title":"Carf Antibody \/ Collaborator of ARF \/ Cdkn2aip","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCarf Antibody \/ Collaborator of ARF \/ Cdkn2aip is a anti-ARF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCarf antibody detects Collaborator of ARF, a nuclear protein encoded by the CDKN2AIP gene on chromosome 4q35.1. CARF (CDKN2A-interacting protein) acts as a transcriptional co-regulator and cell cycle checkpoint factor that mediates the tumor suppressor function of p14ARF and p53. It plays key roles in cellular senescence, DNA damage response, and cell proliferation control. CARF is expressed in most proliferative tissues, including liver, kidney, and hematopoietic cells, with particularly high levels in dividing and stress-activated cells.\u003cbr\u003e\u003cbr\u003eStructurally, CARF contains a coiled-coil domain and nuclear localization signals that facilitate interaction with chromatin-associated proteins and transcriptional regulators. It belongs to the CDKN2A-interacting protein family and acts as a bridge between the ARF tumor suppressor pathway and p53 signaling. CARF directly binds p14ARF and modulates p53 stability through MDM2 regulation, coordinating cell cycle arrest in response to genotoxic stress. Co-localization studies show CARF within the nucleus and nucleolus, where it associates with replication machinery and chromatin-modifying complexes.\u003cbr\u003e\u003cbr\u003eFunctionally, CARF serves as a cell cycle regulator that maintains p53 activity and prevents uncontrolled proliferation. Under DNA damage conditions, CARF enhances p53-dependent transcription of target genes such as CDKN1A (p21), promoting cell cycle arrest and senescence. It also regulates replication stress responses by interacting with checkpoint proteins such as ATR and CHK1. CARF expression levels determine whether a cell undergoes reversible arrest or permanent senescence, integrating multiple stress and growth signals.\u003cbr\u003e\u003cbr\u003eDysregulation of CARF expression has been implicated in cancer and aging. Overexpression can suppress proliferation and induce senescence, while loss of CARF may impair p53 function and enhance oncogenic transformation. In certain tumors, CARF mislocalization correlates with altered cell cycle control. Pathway associations include p53 signaling, DNA damage response, and cell cycle checkpoint regulation. Developmentally, CARF contributes to tissue homeostasis by regulating stem and progenitor cell proliferation.\u003cbr\u003e\u003cbr\u003eThe Carf antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324082541,"sku":"FY13379","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_92fe74da-487c-4bf6-a599-8aa26a10423e.jpg?v=1782237107","url":"https:\/\/www.ebiohippo.com\/products\/carf-antibody-collaborator-of-arf-cdkn2aip-bha17136281","provider":"BioHippo","version":"1.0","type":"link"}