{"product_id":"cas9-expressing-lx-2-stable-cell-line-bhc10923373","title":"Cas9 Expressing LX-2 Stable Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe Cas9 Expressing LX-2 Stable Cell Line is a human hepatic stellate cell model engineered to stably express the Cas9 nuclease, enabling efficient CRISPR-mediated gene editing. This stable line maintains the characteristic LX-2 phenotype while providing a reliable platform for functional genomics, fibrogenesis studies, and targeted gene knockout experiments.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Cas9 Expressing LX-2 Stable Cell Line is supplied as an engineered cell line derived from Human liver.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, Epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). To thaw cells use: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivateOnce cells are attached and recovered use: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 2% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate0.4 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Note: Do not culture LX‑2 cells long-term in 10% FBS; use 2% FBS for routine maintenance to preserve a quiescent-like phenotype and prevent activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in liver biology, metabolism, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eIts consistent growth and validated Cas9 expression make it ideal for liver disease research, drug screening, and mechanistic investigations.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). To thaw cells use: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivateOnce cells are attached and recovered use: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 2% FBS (*Regular) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate0.4 µg\/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. Note: Do not culture LX‑2 cells long-term in 10% FBS; use 2% FBS for routine maintenance to preserve a quiescent-like phenotype and prevent activation.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:2-1:3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 48-96\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180555100525,"sku":"T7727","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/m4N1tndJ831O5eWBBvrhS5pHNHgmNGlgmpq1rgmv.png?v=1774957891","url":"https:\/\/www.ebiohippo.com\/products\/cas9-expressing-lx-2-stable-cell-line-bhc10923373","provider":"BioHippo","version":"1.0","type":"link"}