{"product_id":"casp3-antibody-caspase-3-bha17136148","title":"CASP3 Antibody \/ Caspase 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCASP3 Antibody \/ Caspase 3 is a anti-CASP3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30C96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CASP3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30C96, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCASP3 antibody detects Caspase 3, encoded by the CASP3 gene. Caspase 3 is a cysteine aspartic protease that functions as an executioner caspase in apoptosis. Activated downstream of initiator caspases, Caspase 3 cleaves a broad spectrum of cellular substrates, leading to programmed cell death. CASP3 antibody provides researchers with an essential tool for investigating apoptosis, cancer biology, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eCaspase 3 is synthesized as an inactive zymogen that undergoes proteolytic cleavage during apoptosis to generate the active enzyme. Research using CASP3 antibody has demonstrated that activated Caspase 3 cleaves structural proteins, DNA repair enzymes, and signaling molecules, dismantling cellular architecture and promoting apoptotic progression. Its activity is a hallmark of programmed cell death and is widely used as a biomarker of apoptosis in experimental systems.\u003cbr\u003e\u003cbr\u003eIn cancer research, studies with CASP3 antibody have revealed that impaired Caspase 3 activation contributes to resistance to chemotherapy and radiation therapy. Tumors that downregulate or inactivate Caspase 3 evade apoptosis, enhancing survival and proliferation. Conversely, forced activation of Caspase 3 promotes cell death and tumor regression. These observations highlight Caspase 3 as a potential therapeutic target and prognostic marker in oncology.\u003cbr\u003e\u003cbr\u003eCaspase 3 also plays critical roles in neurodegeneration. Research using CASP3 antibody has shown that aberrant Caspase 3 activity contributes to neuronal loss in diseases such as Alzheimer's, Parkinson's, and Huntington's. Excessive activation promotes neuronal apoptosis, while inhibition has been explored as a neuroprotective strategy. Beyond the nervous system, Caspase 3 regulates apoptosis in immune cells, cardiac cells, and developmental processes, reflecting its broad biological importance.\u003cbr\u003e\u003cbr\u003eCASP3 antibody is widely used in western blotting, immunohistochemistry, flow cytometry, and ELISA. Western blotting distinguishes full length and cleaved forms, immunohistochemistry detects apoptotic cells in tissue, flow cytometry quantifies apoptosis in cell populations, and ELISA measures Caspase 3 activity in lysates. These applications make CASP3 antibody indispensable for apoptosis research.\u003cbr\u003e\u003cbr\u003eBy supplying validated CASP3 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CASP3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CASP3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CASP3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CASP3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CASP3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047319232877,"sku":"FY13246","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_806b50e7-e8ce-4488-acc3-fde945210d6c.jpg?v=1772019415","url":"https:\/\/www.ebiohippo.com\/products\/casp3-antibody-caspase-3-bha17136148","provider":"BioHippo","version":"1.0","type":"link"}