{"product_id":"cattle-ctnt-tnnt2-troponin-t-type-2-cardiac-elisa-kit-bhe15208985","title":"Cattle cTnT\/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ecTnT\/TNNT2 (Troponin T Type 2, Cardiac)\u003c\/strong\u003e is a biologically relevant protein marker measured to support mechanistic studies and biomarker discovery (context dependent).\u003c\/p\u003e\u003cp\u003eProtein concentrations can change due to secretion, degradation, cell composition shifts, or post-transcriptional regulation, so ELISA readouts often add information beyond gene expression alone.\u003c\/p\u003e\u003cp\u003eQuantitative measurements help compare groups and time points using standardized curves and can be interpreted alongside phenotype and pathway-specific readouts.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ecTnT\/TNNT2 (Troponin T Type 2, Cardiac)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eCattle\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT\/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT\/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT\/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT\/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 78.13-5000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 26.1 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966026772845,"sku":"ELK0100-96T","price":764.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966026805613,"sku":"ELK0100-48T","price":535.6,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966026838381,"sku":"ELK0100-96TX5","price":3248.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_ef577b76-4463-4b4e-88d3-ee059455d5d4.jpg?v=1771845916","url":"https:\/\/www.ebiohippo.com\/products\/cattle-ctnt-tnnt2-troponin-t-type-2-cardiac-elisa-kit-bhe15208985","provider":"BioHippo","version":"1.0","type":"link"}