{"product_id":"cattle-tgfb1-transforming-growth-factor-beta-1-elisa-kit-bhe15200621","title":"Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eTGFb1 (Transforming Growth Factor Beta 1)\u003c\/strong\u003e is a growth factor–related marker involved in cellular proliferation, differentiation, or tissue remodeling processes.\u003c\/p\u003e\u003cp\u003eGrowth factor concentrations can reflect changes in tissue repair, fibrosis, angiogenesis, or tumor microenvironment signaling depending on the specific target and model.\u003c\/p\u003e\u003cp\u003eBecause many growth factors act locally and are regulated post-transcriptionally, protein quantification can add clarity beyond mRNA-only measurements.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eTGFb1 (Transforming Growth Factor Beta 1)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eCattle\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, platelet-poor plasma, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 4.8 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 2h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52964576330093,"sku":"ELK5467-96T","price":764.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52964576362861,"sku":"ELK5467-48T","price":535.6,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52964576395629,"sku":"ELK5467-96TX5","price":3248.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_a8470752-ac65-4871-8dce-cfbfb2e72ccb.jpg?v=1771840835","url":"https:\/\/www.ebiohippo.com\/products\/cattle-tgfb1-transforming-growth-factor-beta-1-elisa-kit-bhe15200621","provider":"BioHippo","version":"1.0","type":"link"}