{"product_id":"cbx6-antibody-chromobox-protein-homolog-6-bha17135832","title":"CBX6 Antibody \/ Chromobox protein homolog 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCBX6 Antibody \/ Chromobox protein homolog 6 is a anti-CBX6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CBX6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCBX6 antibody detects Chromobox protein homolog 6, a member of the chromobox family within the Polycomb group (PcG) proteins, which play a critical role in transcriptional repression and chromatin remodeling. The full UniProt recommended name is Chromobox protein homolog 6 (CBX6), with older or alternate designations including chromobox homolog 6, Polycomb group protein chromobox 6, and human homolog of Drosophila Polycomb protein. CBX6 is a nuclear protein involved in maintaining the repressed state of genes through the formation of polycomb repressive complexes (PRCs), particularly PRC1, which works in conjunction with PRC2 to regulate histone methylation states and gene silencing across development and cell differentiation.\u003cbr\u003e\u003cbr\u003eCBX6 binds to trimethylated histone H3 at lysine 27 (H3K27me3), a histone modification mark associated with gene repression, serving as a reader of histone methylation marks and recruiting additional factors to maintain chromatin compaction. It contributes to establishing heritable transcriptional repression, ensuring stable lineage-specific expression patterns in embryonic stem cells and adult tissues. Dysregulation of CBX6 antibody targets, particularly in the Polycomb family, has been associated with aberrant cell proliferation and oncogenic transformation. Overexpression or misexpression of CBX6 has been observed in several cancer types, including hepatocellular carcinoma, prostate cancer, and gliomas, where it may influence epithelial-mesenchymal transition (EMT) and cancer stemness features.\u003cbr\u003e\u003cbr\u003eIn molecular biology and epigenetic research, CBX6 antibody is widely used for immunofluorescence, chromatin immunoprecipitation (ChIP), and western blot assays to examine Polycomb protein localization and function. It helps distinguish CBX6 from other chromobox homologs (CBX2, CBX4, CBX7, CBX8), each of which has distinct but sometimes overlapping genomic binding profiles and developmental roles. CBX6 has been reported to modulate PRC1 recruitment in concert with histone ubiquitination factors such as RNF2 and BMI1, and to act as a chromatin structural modulator linking histone marks to gene repression. The protein is essential in maintaining pluripotency, regulating HOX gene clusters, and ensuring proper cell fate decisions.\u003cbr\u003e\u003cbr\u003eCBX6's gene, CBX6, is located on chromosome 22q13.1. It encodes a protein of approximately 412 amino acids, containing a conserved chromodomain responsible for histone binding and a C-terminal region that mediates protein-protein interactions within the PRC complex. Expression is ubiquitous but higher in testis, placenta, and certain cancerous tissues. Loss-of-function mutations or altered methylation of the CBX6 promoter region can disrupt PRC targeting, influencing gene activation programs and tumor suppressor networks. Studies also show CBX6 modulates long noncoding RNA (lncRNA)-mediated gene silencing, including interactions with lncRNAs such as HOTAIR and MALAT1, further linking CBX6 to chromatin topology and transcriptional memory.\u003cbr\u003e\u003cbr\u003eBecause of its functional importance, CBX6 serves as a key biomarker in epigenetic and oncogenic signaling studies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CBX6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CBX6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CBX6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CBX6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CBX6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306453357,"sku":"FY12930","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0840d91e-f782-4d0f-914f-32ce0d7b1468.jpg?v=1782237055","url":"https:\/\/www.ebiohippo.com\/products\/cbx6-antibody-chromobox-protein-homolog-6-bha17135832","provider":"BioHippo","version":"1.0","type":"link"}