{"product_id":"cby2-antibody-chibby-2-bha17135639","title":"CBY2 Antibody \/ Chibby 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCBY2 Antibody \/ Chibby 2 is a anti-CBY2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CBY2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCBY2 antibody detects Chibby homolog 2, a centrosome- and cilium-associated protein involved in ciliogenesis, cell polarity, and Wnt signaling regulation. Encoded by the CBY2 gene on chromosome 12q24.13, this protein belongs to the Chibby family, which includes CBY1 and CBY3, known for modulating beta-catenin signaling and ciliary structure. CBY2 contains coiled-coil domains that mediate dimerization and interaction with other ciliary proteins such as CEP164 and ODF2. It localizes to the basal body and transition zone of cilia, where it contributes to the assembly and stabilization of the ciliary axoneme.\u003cbr\u003e\u003cbr\u003eCBY2 is expressed in ciliated tissues including respiratory epithelium, reproductive organs, and brain ependyma. It plays an essential role in the early stages of ciliogenesis by promoting docking of basal bodies to the plasma membrane and initiating axoneme extension. Disruption of CBY2 function leads to defective ciliogenesis, altered epithelial polarity, and impaired signaling through cilia-dependent pathways such as Hedgehog and Wnt. In reproductive cells, CBY2 contributes to flagellar formation and sperm motility, while in neurons it supports ependymal cilia function crucial for cerebrospinal fluid flow.\u003cbr\u003e\u003cbr\u003eThe CBY2 antibody is widely used in cell biology and developmental studies to analyze ciliary formation, centrosomal organization, and beta-catenin signaling. Western blot analysis detects a 42 kilodalton band corresponding to full-length CBY2, and immunofluorescence reveals punctate staining at basal bodies marked by gamma-tubulin. In cilia-deficient models, CBY2 expression serves as a marker for disrupted ciliogenesis. The antibody is also useful for exploring CBY2's role in planar cell polarity and coordination of multiciliated cell differentiation.\u003cbr\u003e\u003cbr\u003eMutations or reduced expression of CBY2 have been linked to ciliopathies, infertility, and hydrocephalus, reflecting its vital role in ciliary assembly. The CBY2 antibody provides a powerful tool for investigating these disorders and for mapping ciliary protein networks in epithelial and germline systems.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CBY2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CBY2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CBY2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CBY2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CBY2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297933677,"sku":"FY12736","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c840937c-907b-48df-b3a8-538cc7b7e49f.jpg?v=1782237036","url":"https:\/\/www.ebiohippo.com\/products\/cby2-antibody-chibby-2-bha17135639","provider":"BioHippo","version":"1.0","type":"link"}