{"product_id":"ccl4-antibody-bha10537952","title":"CCL4 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-CCL4 antibody raised in Rabbit, with confirmed utility in WB, ELISA. It is designed to detect CCL4 protein in Human and supports researchers working in immunology contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthesized peptide derived from the Internal region of Human MIP-1β. — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCCL4\u003c\/strong\u003e (also referred to as MIP 1 beta, \tSecreted protein G 26, ACT 2, ACT-2, ACT2, AT744.1, AT744.2, C C motif chemokine 4, C C motif chemokine 4 like, C C motif chemokine ligand 4 like 1, C C motif chemokine ligand 4 like 2, CC chemokine ligand 4, CC chemokine ligand 4L1, CC chemokine ligand 4L1d2, CC chemokine ligand 4L2, CCL4, CCL4_HUMAN, ccl4l 1, CCL4L, CCL4L1, Chemokine (C C motif) ligand 4, Chemokine (C C motif) ligand 4 like 1, Chemokine (C C motif) ligand 4 like 1, telomeric, Chemokine (C C motif) ligand 4 like 2, Chemokine CC Motif Ligand 4, G 26, G 26 T lymphocyte secreted protein, G-26 T-lymphocyte-secreted protein, HC21, Immune activation 2, LAG 1, LAG-1, LAG1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1 beta, Macrophage inflammatory protein 1-beta, Macrophage inflammatory protein 1b2, MGC104418, MGC126025, MGC126026, MIP-1-beta, MIP-1-beta(1-69), MIP-1-beta(3-69), MIP1 beta, MIP1B, MIP1B1, Monocyte adherence induced protein 5 alpha, PAT 744, Protein H400, SCYA2, SCYA4, SCYA4L, SCYA4L1, SCYA4L2, SCYQ4L2, Secreted protein G 26, Secreted protein G26, SIS gamma, SIS-gamma, Small inducible cytokine A4 (homologous to mouse Mip 1b), Small inducible cytokine A4, small inducible cytokine A4-like, Small-inducible cytokine A4, T cell activation protein 2, T-cell activation protein 2) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CCL4 biology, including known isoforms, interactors, and disease-relevant expression patterns in immunology.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e Western blot confirms target molecular weight and distinguishes isoforms under denaturing conditions. Expected band size should align with reported MW.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53245518741869,"sku":"CSB-PA005136-50UG","price":119.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53245625205101,"sku":"CSB-PA005136-100UG","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA005136-WB-1.jpg?v=1776753175","url":"https:\/\/www.ebiohippo.com\/products\/ccl4-antibody-bha10537952","provider":"BioHippo","version":"1.0","type":"link"}