{"product_id":"ccl4-antibody-fitc-conjugated-bha10521754","title":"CCL4 Antibody, FITC conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-CCL4 antibody raised in Rabbit, conjugated with FITC. It is designed to detect CCL4 protein in Human and supports researchers working in immunology contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human C-C motif chemokine 4 protein (24-84AA) — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFITC conjugate:\u003c\/strong\u003e Direct fluorescence detection (Ex ~490\/Em ~525 nm). FITC is photolabile; protect samples and use antifade reagents for slides.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eCCL4\u003c\/strong\u003e (also referred to as MIP 1 beta, \tSecreted protein G 26, ACT 2, ACT-2, ACT2, AT744.1, AT744.2, C C motif chemokine 4, C C motif chemokine 4 like, C C motif chemokine ligand 4 like 1, C C motif chemokine ligand 4 like 2, CC chemokine ligand 4, CC chemokine ligand 4L1, CC chemokine ligand 4L1d2, CC chemokine ligand 4L2, CCL4, CCL4_HUMAN, ccl4l 1, CCL4L, CCL4L1, Chemokine (C C motif) ligand 4, Chemokine (C C motif) ligand 4 like 1, Chemokine (C C motif) ligand 4 like 1, telomeric, Chemokine (C C motif) ligand 4 like 2, Chemokine CC Motif Ligand 4, G 26, G 26 T lymphocyte secreted protein, G-26 T-lymphocyte-secreted protein, HC21, Immune activation 2, LAG 1, LAG-1, LAG1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1 beta, Macrophage inflammatory protein 1-beta, Macrophage inflammatory protein 1b2, MGC104418, MGC126025, MGC126026, MIP-1-beta, MIP-1-beta(1-69), MIP-1-beta(3-69), MIP1 beta, MIP1B, MIP1B1, Monocyte adherence induced protein 5 alpha, PAT 744, Protein H400, SCYA2, SCYA4, SCYA4L, SCYA4L1, SCYA4L2, SCYQ4L2, Secreted protein G 26, Secreted protein G26, SIS gamma, SIS-gamma, Small inducible cytokine A4 (homologous to mouse Mip 1b), Small inducible cytokine A4, small inducible cytokine A4-like, Small-inducible cytokine A4, T cell activation protein 2, T-cell activation protein 2) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CCL4 biology, including known isoforms, interactors, and disease-relevant expression patterns in immunology.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRefer to product documentation for confirmed application data and recommended conditions.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241336529261,"sku":"CSB-PA05949C0Rb-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241437159789,"sku":"CSB-PA05949C0Rb-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_f08ea4e1-9897-4a53-9db4-d2e5e8ef320a.jpg?v=1784927139","url":"https:\/\/www.ebiohippo.com\/products\/ccl4-antibody-fitc-conjugated-bha10521754","provider":"BioHippo","version":"1.0","type":"link"}