{"product_id":"ccl5-antibody-pair-bha10575605","title":"CCL5 Antibody Pair","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a matched capture and detection antibody pair designed for sandwich ELISA quantification of C-C motif chemokine 5 in Human,Mouse,Guinea pig,Bovine,Pig,Dog,Rabbit,Goat,Sheep. The pair consists of a capture antibody (unconjugated) and a detection antibody (biotin-conjugated), both raised in Capture: Rabbit | Detection: Rabbit, enabling a complete sandwich format without the need for additional antibody sourcing.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClonality:\u003c\/strong\u003e Capture: Polyclonal | Detection: Polyclonal — each component is independently validated for capture and detection roles in the sandwich format.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugation format:\u003c\/strong\u003e Capture: Unconjugated | Detection: Biotin conjugated — the detection antibody carries the biotin label for streptavidin-amplified signal generation; the capture antibody is unconjugated for plate coating.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype:\u003c\/strong\u003e Capture: IgG | Detection: IgG — matching isotype across capture and detection components reduces non-specific background from secondary reagents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication notes:\u003c\/strong\u003e We recommend using the capture antibody at a concentration of 1.4ug\/ml and the detection antibody at a concentration of 1ug\/ml.Optimal dilutions should be determined experimentally by the researcher.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eC-C motif chemokine 5\u003c\/strong\u003e (also referred to as EoCP,Eosinophil chemotactic cytokine,SIS-delta,Small-inducible cytokine A5,T cell-specific protein P228,TCP228,T-cell-specific protein RANTES,CCL5,D17S136E, SCYA5) is a protein target of interest in Human,Mouse,Guinea pig,Bovine,Pig,Dog,Rabbit,Goat,Sheep systems. Researchers studying C-C motif chemokine 5 typically rely on quantitative sandwich ELISA to measure protein levels in serum, plasma, cell culture supernatants, or tissue homogenates. Consult UniProt, NCBI Gene, and primary literature for current annotation of C-C motif chemokine 5 expression, function, and disease relevance.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSandwich ELISA quantification:\u003c\/strong\u003e The matched pair is optimized for standard sandwich ELISA formats. The capture antibody is coated to the plate surface; after sample incubation, the biotinylated detection antibody is added, followed by streptavidin-HRP and colorimetric substrate for quantification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample types:\u003c\/strong\u003e Typically validated for serum, plasma, and cell culture supernatants. Confirm sample type compatibility with the product datasheet before use in other matrices.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental design\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody concentrations:\u003c\/strong\u003e We recommend using the capture antibody at a concentration of 1.4ug\/ml and the detection antibody at a concentration of 1ug\/ml.Optimal dilutions should be determined experimentally by the researcher.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStandard curve:\u003c\/strong\u003e Use recombinant C-C motif chemokine 5 protein as a standard for absolute quantification; confirm standard purity and activity before use.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Species reactivity is reported for Human,Mouse,Guinea pig,Bovine,Pig,Dog,Rabbit,Goat,Sheep. Use in other species matrices requires empirical confirmation of antibody–antigen binding.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStreptavidin-HRP:\u003c\/strong\u003e Select a high-quality streptavidin-HRP conjugate and optimize its concentration to minimize background while maintaining signal sensitivity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"1 Pair (5*96 well plates)","offer_id":53247433015661,"sku":"CSB-EAP05955","price":1000.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/ccl5-antibody-pair-bha10575605","provider":"BioHippo","version":"1.0","type":"link"}