{"product_id":"cd116-antibody-csf2ra-colony-stimulating-factor-2-receptor-alpha-bha17136187","title":"CD116 Antibody \/ CSF2RA \/ Colony stimulating factor 2 receptor alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCD116 Antibody \/ CSF2RA \/ Colony stimulating factor 2 receptor alpha is a anti-CD116 Rabbit antibody Recombinant Rabbit Monoclonal clone 31C12 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD116\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31C12, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCD116 antibody detects Colony stimulating factor 2 receptor alpha, encoded by the CSF2RA gene. Colony stimulating factor 2 receptor alpha is the alpha chain of the receptor for granulocyte macrophage colony stimulating factor, a cytokine that regulates growth, differentiation, and activation of hematopoietic cells. CD116 antibody provides researchers with a valuable reagent to study immune cell proliferation, myeloid biology, and cytokine signaling.\u003cbr\u003e\u003cbr\u003eColony stimulating factor 2 receptor alpha pairs with a common beta chain, shared with IL-3 and IL-5 receptors, to form a functional heterodimeric receptor. Research using CD116 antibody has demonstrated that ligand binding initiates dimerization and activation of JAK2, which then phosphorylates downstream signaling proteins including STAT5. This signaling cascade regulates survival, proliferation, and activation of granulocytes, macrophages, and eosinophils. The alpha chain provides ligand specificity and determines receptor affinity for granulocyte macrophage colony stimulating factor.\u003cbr\u003e\u003cbr\u003eStudies with CD116 antibody have revealed that disruption of CSF2RA function impairs myeloid cell development and immune defense. Loss of function mutations cause X linked pulmonary surfactant metabolism dysfunction, reflecting impaired alveolar macrophage activity. This highlights the critical role of Colony stimulating factor 2 receptor alpha in lung homeostasis and host defense. Conversely, overactivation of the receptor pathway has been implicated in inflammatory diseases, such as asthma and autoimmune disorders.\u003cbr\u003e\u003cbr\u003eIn cancer biology, CD116 has been linked to leukemia and myelodysplastic syndromes. Research using CD116 antibody has shown that abnormal signaling through the CSF2RA pathway contributes to malignant transformation and proliferation of hematopoietic cells. Because of this, receptor signaling is being explored as a therapeutic target in hematologic malignancies. Monitoring expression with CD116 antibody provides important diagnostic and prognostic information.\u003cbr\u003e\u003cbr\u003eCD116 antibody is widely used in flow cytometry, immunohistochemistry, and western blotting. Flow cytometry quantifies receptor expression on myeloid subsets, immunohistochemistry localizes receptor distribution in tissues, and western blotting confirms receptor size and regulation. These applications make CD116 antibody a versatile tool in immunology and hematology research.\u003cbr\u003e\u003cbr\u003eBy providing validated CD116 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD116 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD116 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD116 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD116 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD116+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047321166189,"sku":"FY13285","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7bbe8991-bfa2-49f0-b195-78e7309aee43.jpg?v=1772019423","url":"https:\/\/www.ebiohippo.com\/products\/cd116-antibody-csf2ra-colony-stimulating-factor-2-receptor-alpha-bha17136187","provider":"BioHippo","version":"1.0","type":"link"}