{"product_id":"cd11a-antibody-lfa-1-bha17106008","title":"CD11a Antibody \/ LFA-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCD11a Antibody \/ LFA-1 is a research-use-only Rabbit polyclonal (rabbit origin) Rabbit IgG directed against \u003cstrong\u003eCD11a \/ LFA-1\u003c\/strong\u003e. It is supplied for interpretation-focused detection and comparative profiling in WB, FACS, ICC. \u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget context:\u003c\/strong\u003e This antibody is raised against \u003cem\u003eAmino acids F161-L349 from the human protein were used as the immunogen for the CD11a antibody.\u003c\/em\u003e. Epitope context matters because isoforms, processing, and post-translational modifications can change what is accessible in a given assay.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified. Format influences background and compatibility with different detection chemistries; conjugated formats (when present) can simplify multiplexing and reduce reliance on secondary reagents.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human. Cross-species performance can vary with sequence divergence and epitope conservation, so interpretation should be anchored with appropriate biological controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e WB, FACS, ICC. These indicate assay contexts where the antibody is commonly applied; actual performance depends on sample type and processing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLimitations:\u003c\/strong\u003e This CD11a antibody is available for research use only.. Consider these constraints when selecting controls and when comparing results across sample matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePolyclonal reagents can differ in how they recognize epitope features. Monoclonal antibodies often provide more consistent epitope targeting across lots, while polyclonal preparations may broaden recognition across related epitope variants. \u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eCD11a \/ LFA-1\u003c\/strong\u003e refers to the gene\/protein target stated in the product record. Protein targets can exhibit context-dependent expression, regulated turnover, isoform diversity, and post-translational modifications that affect apparent molecular weight and epitope accessibility. For curated functional annotation, sequence features, and expression context, consult \u003ca href=\"https:\/\/www.uniprot.org\/uniprotkb\/P20701\/entry\"\u003eUniProtKB P20701\u003c\/a\u003e, \u003ca href=\"https:\/\/www.ensembl.org\/\"\u003eEnsembl\u003c\/a\u003e, and \u003ca href=\"https:\/\/www.proteinatlas.org\/\"\u003eHuman Protein Atlas\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eIntegrating antibody-based detection with single-cell and spatial atlasing efforts to connect RNA programs with protein-level abundance and localization in defined cell states.\u003c\/li\u003e\n\u003cli\u003eExpanding multiplexed imaging and high-content screening, where reagent specificity, cross-reactivity risk, and channel design (including direct conjugates) become central to interpretation.\u003c\/li\u003e\n\u003cli\u003eGrowing emphasis on reproducibility and application-specific validation frameworks (e.g., genetic perturbation controls, orthogonal measurements, and independent antibody strategies) when drawing mechanistic conclusions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e commonly used to compare relative abundance\/size (e.g., band intensity or mobility shifts) between conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFACS:\u003c\/strong\u003e commonly used for qualitative\/quantitative detection where compatible with the assay context.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunocytochemistry (ICC):\u003c\/strong\u003e commonly used to compare cellular distribution and cell-to-cell heterogeneity in cultured cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpretation typically focuses on relative differences (presence\/absence, fold-changes, compartment shifts, or population-level shifts) rather than absolute quantitation. When signal changes are observed, they may reflect altered expression, altered localization\/trafficking, changes in modification state, or differences in sample composition; orthogonal readouts and appropriate controls help distinguish these possibilities.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication details (record-specific):\u003c\/strong\u003e Western blot: 0.5-1ug\/ml,Flow Cytometry:1-2ug\/ml,Immunocytochemistry: 1-2ug\/ml\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eApplication notes (record-specific):\u003c\/strong\u003e Optimal dilution of the CD11a antibody should be determined by the researcher.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct description (record-specific):\u003c\/strong\u003e CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePotential confounders:\u003c\/strong\u003e isoforms, proteolytic processing, and PTMs can change epitope presentation and apparent size; fixation\/denaturation state can also expose or mask epitopes. Species differences near the epitope may affect cross-reactivity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include genetic perturbation (KO\/KD) or overexpression comparisons, orthogonal measurement (e.g., transcript or proteomics), and independent antibody\/epitope strategies. For conjugated reagents, include staining-only\/background controls appropriate to the detection chemistry.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eImmunogen\/epitope context is described as: \u003cem\u003eAmino acids F161-L349 from the human protein were used as the immunogen for the CD11a antibody.\u003c\/em\u003e. Monoclonal and polyclonal formats differ in epitope breadth; this can influence sensitivity to sequence variants, isoforms, or PTM-dependent recognition.\u003c\/p\u003e\n\u003c!-- Sources (internal):\n- UniProtKB entry P20701 (UniProt Consortium): https:\/\/www.uniprot.org\/uniprotkb\/P20701\/entry\n- Ensembl genome browser (EMBL-EBI \/ Wellcome Sanger): https:\/\/www.ensembl.org\/\n- The Human Protein Atlas (KTH \/ SciLifeLab): https:\/\/www.proteinatlas.org\/\n- A proposal for validation of antibodies (Uhlén et al., Nature Methods, 2016): https:\/\/www.nature.com\/articles\/nmeth.3995.pdf\n- Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents (Bradbury \u0026 Plückthun, PEDS, 2015): https:\/\/academic.oup.com\/peds\/article\/28\/10\/303\/1478378\n- Standardize antibodies used in research (Bradbury, Plückthun et al., Nature, 2015): https:\/\/www.nature.com\/articles\/518027a.pdf\n--\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53210328072557,"sku":"R32698","price":462.47,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a9c79b3f-2cea-47c5-9ce2-77e20853d053.jpg?v=1775785795","url":"https:\/\/www.ebiohippo.com\/products\/cd11a-antibody-lfa-1-bha17106008","provider":"BioHippo","version":"1.0","type":"link"}