{"product_id":"cd160-nfat-reporter-lentivirus-bhv19400265","title":"CD160\/NFAT Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD160 is a glycosylphosphatidylinositol-anchored cell surface receptor expressed predominantly on natural killer cells and subsets of T cells. On NK cells, engagement of CD160 by its ligands, including major histocompatibility complex class I molecules and herpesvirus entry mediator, can deliver activating signals that enhance cytotoxicity and cytokine production. CD160 signaling feeds into the calcium-dependent NFAT pathway, a key transcriptional program downstream of immunoreceptor engagement that controls effector gene expression. Depending on cellular context, CD160 has also been described as a co-inhibitory receptor, reflecting its complex role in immune regulation. The receptor is studied for its contributions to NK and T cell activation, immune surveillance, and as a candidate target in immunotherapy.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD160\/NFAT Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human CD160 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NFAT response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to CD160 engagement with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of NK cell activation, immune responses, and CD160-targeting immunotherapies in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CD160 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616702829,"sku":"TRV-0017-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_905d87d2-8d59-4bfd-b6cd-ce2d45ed621b.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/cd160-nfat-reporter-lentivirus-bhv19400265","provider":"BioHippo","version":"1.0","type":"link"}