{"product_id":"cd163-antibody-bha17135536","title":"CD163 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCD163 Antibody is a anti-CD163 Rabbit antibody Recombinant Rabbit Monoclonal clone 32C15 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD163\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C15, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCD163 antibody detects CD163, a scavenger receptor encoded by the CD163 gene and expressed predominantly on monocytes and macrophages. CD163 belongs to the scavenger receptor cysteine rich superfamily and acts as a hemoglobin-haptoglobin receptor. By binding hemoglobin-haptoglobin complexes, CD163 mediates their clearance, preventing oxidative damage and promoting anti-inflammatory responses.\u003cbr\u003e\u003cbr\u003eCD163 antibody is widely applied in immunology, hematology, and inflammation research. CD163 expression serves as a marker of alternatively activated macrophages, often associated with tissue repair, immune suppression, and tumor progression. By detecting CD163, researchers can assess macrophage polarization and function in contexts such as sepsis, atherosclerosis, cancer, and chronic inflammation.\u003cbr\u003e\u003cbr\u003e Flow cytometry with CD163 antibody distinguishes macrophage populations based on activation state. Immunohistochemistry maps CD163 expression in tissues including liver, spleen, and tumors, while immunofluorescence highlights surface localization on macrophages. Western blotting provides complementary confirmation of expression levels. These applications make CD163 antibody versatile for both basic and translational research.\u003cbr\u003e\u003cbr\u003eCD163 has clinical relevance as a biomarker for inflammation and disease activity. Soluble CD163, shed from the cell surface, is detectable in plasma and serves as a marker of macrophage activation. Elevated levels correlate with disease severity in inflammatory conditions such as rheumatoid arthritis, lupus, and sepsis. By applying CD163 antibody, scientists can evaluate macrophage biology and disease mechanisms.\u003cbr\u003e\u003cbr\u003eBeyond immunology, CD163 contributes to iron metabolism by mediating hemoglobin clearance. It also influences tumor microenvironments by supporting immune evasion. These diverse functions emphasize the broad significance of CD163 antibody in biology and medicine.\u003cbr\u003e\u003cbr\u003eCD163 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD163 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD163 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD163 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD163 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD163+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047295017325,"sku":"FY12633","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cbfc5970-8ad6-49ef-b311-4e5e339f2d1c.jpg?v=1772019318","url":"https:\/\/www.ebiohippo.com\/products\/cd163-antibody-bha17135536","provider":"BioHippo","version":"1.0","type":"link"}