{"product_id":"cd163-antibody-bha17136168","title":"CD163 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCD163 Antibody is a anti-CD163 Rabbit antibody Recombinant Rabbit Monoclonal clone 32C14 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD163\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C14, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCD163 antibody detects Cluster of Differentiation 163, encoded by the CD163 gene. CD163 is a scavenger receptor expressed primarily on monocytes and macrophages, where it mediates endocytosis of hemoglobin-haptoglobin complexes and regulates inflammatory responses. CD163 antibody provides researchers with a valuable tool to study macrophage biology, innate immunity, and inflammation.\u003cbr\u003e\u003cbr\u003eCluster of Differentiation 163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine rich superfamily. Research using CD163 antibody has shown that it binds hemoglobin-haptoglobin complexes with high affinity, promoting their clearance from circulation. This function prevents oxidative damage and inflammation caused by free hemoglobin. By regulating hemoglobin metabolism, CD163 protects tissues from injury following hemolysis.\u003cbr\u003e\u003cbr\u003eStudies with CD163 antibody have revealed additional roles in immune regulation. CD163 functions as an anti-inflammatory receptor by triggering secretion of anti-inflammatory cytokines such as IL-10 upon ligand binding. Shedding of CD163 from the cell surface produces soluble CD163, which circulates in plasma and serves as a biomarker of macrophage activation and inflammation. Elevated soluble CD163 levels are observed in conditions such as sepsis, liver disease, and autoimmune disorders.\u003cbr\u003e\u003cbr\u003eCD163 expression is tightly regulated by cytokines and environmental signals. Research using CD163 antibody has demonstrated that IL-6 and glucocorticoids induce expression, while pro-inflammatory stimuli such as lipopolysaccharide downregulate it. This dynamic regulation reflects the role of CD163 in resolving inflammation and restoring homeostasis.\u003cbr\u003e\u003cbr\u003eIn pathology, CD163 has emerged as a biomarker of tumor-associated macrophages. Studies with CD163 antibody have shown that high CD163 expression correlates with immunosuppressive macrophage populations in cancers, supporting tumor progression and poor prognosis. In cardiovascular disease, altered CD163 levels are linked to atherosclerosis and vascular inflammation. These findings underscore the importance of CD163 in both protective and pathological processes.\u003cbr\u003e\u003cbr\u003eCD163 antibody is widely applied in flow cytometry, immunohistochemistry, and western blotting. Flow cytometry quantifies macrophage subsets, immunohistochemistry identifies tissue distribution, and western blotting confirms protein size and expression. These applications make CD163 antibody indispensable for immunology and translational research.\u003cbr\u003e\u003cbr\u003eBy providing validated CD163 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD163 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD163 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD163 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD163 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD163+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047320314221,"sku":"FY13266","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/cd163-antibody-bha17136168","provider":"BioHippo","version":"1.0","type":"link"}