{"product_id":"cd163-antibody-bha17136353","title":"Cd163 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCd163 Antibody is a anti-CD163 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cell surface, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD163\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCd163 is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on cells of the monocyte\/macrophage lineage, where it functions as a hemoglobin-haptoglobin complex receptor. By mediating clearance of hemoglobin from sites of tissue damage or intravascular hemolysis, Cd163 plays a central role in protecting tissues from oxidative stress and inflammation. A Cd163 antibody is frequently used to study macrophage biology, immune regulation, and tissue remodeling in both mouse and rat models.    \u003cbr\u003e\u003cbr\u003e  Cd163 expression is strongly associated with alternatively activated (M2) macrophages, which are involved in anti-inflammatory processes, tissue repair, and immunosuppression. This makes Cd163 a valuable marker for identifying macrophage polarization states. Employing a Cd163 antibody allows researchers to investigate the dynamics of macrophage activation in response to injury, infection, or disease progression.    \u003cbr\u003e\u003cbr\u003e  Beyond its role in hemoglobin clearance, Cd163 has broader immunomodulatory functions. Engagement of Cd163 can trigger anti-inflammatory signaling cascades and promote the release of heme oxygenase-1, further contributing to cytoprotection. Dysregulated Cd163 expression has been implicated in conditions such as sepsis, atherosclerosis, and chronic inflammatory diseases. In cancer, Cd163-positive macrophages are often linked to tumor-associated macrophages (TAMs), which can support tumor growth and immune evasion. A Cd163 antibody is therefore a powerful tool in both basic immunology and translational research.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD163 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD163 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD163 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD163 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD163+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326081389,"sku":"RQ8935","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b800bed3-f24c-4001-8c2c-0eb6c7ea011b.jpg?v=1772019448","url":"https:\/\/www.ebiohippo.com\/products\/cd163-antibody-bha17136353","provider":"BioHippo","version":"1.0","type":"link"}