{"product_id":"cd16a-nfat-reporter-lentivirus-adcc-assay-bhv19400252","title":"CD16A\/NFAT Reporter Lentivirus (ADCC Assay)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD16A (FcγRIIIa) is a low-affinity Fc receptor for immunoglobulin G expressed on natural killer cells and other effector cells. By engaging the Fc region of antibodies bound to a target cell, CD16A triggers antibody-dependent cellular cytotoxicity (ADCC), a key mechanism by which therapeutic antibodies eliminate tumor and virally infected cells. Receptor engagement raises intracellular calcium and activates the transcription factor NFAT, providing a measurable transcriptional readout of effector-cell activation. A common polymorphism produces high-affinity (V158) and low-affinity (F158) allotypes that influence the strength of ADCC, making CD16A a central focus of antibody-engineering and immuno-oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD16A\/NFAT Reporter Lentivirus is an immunotherapy reporter system for quantifying antibody-dependent cellular cytotoxicity. A receptor lentivirus constitutively expresses the human CD16A high-affinity V158 allotype with antibiotic selection, intended for use alongside a companion low-affinity F158 product as a control. A reporter lentivirus carries NFAT response elements driving a dual reporter, secreted Gaussia luciferase and a fluorescent protein. Sequential transduction and selection generates a dual-stable effector cell line, such as Jurkat, that responds to antibody-coated target cells.\u003c\/p\u003e\n\u003cp\u003eWhen CD16A engages target-bound IgG, intracellular calcium rises and activates NFAT, driving the reporters. Secreted Gaussia luciferase allows kinetic sampling of media without lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped, PEG- and sucrose-gradient-purified particles transduce difficult-to-transfect cells, supporting ADCC potency testing and antibody screening.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human ADCC Assay under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CD16A(V158)-GFP\/NFAT-RFP \/ RFP \/ 2x10^6","offer_id":53251615392109,"sku":"TRV-0002-1S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-RFP\/NFAT-GFP \/ GFP \/ 2x10^6","offer_id":53362085593453,"sku":"TRV-0002-2S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP \/ GFP \/ 2x10^6","offer_id":53362085626221,"sku":"TRV-0002-3S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-RFP \/ RFP \/ 2x10^6","offer_id":53362085658989,"sku":"TRV-0002-4S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP-GLuc \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085691757,"sku":"TRV-0002-6S","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(F158)-BSD\/NFAT-GFP-GLuc (Low Affinity) \/ GFP-P2A-GLuc \/ 2x10^6","offer_id":53362085724525,"sku":"TRV-0002-6N","price":895.0,"currency_code":"USD","in_stock":true},{"title":"CD16A(V158)-BSD\/NFAT-GFP-GLuc Jurkat Cells \/ GFP-P2A-GLuc \/ 5x10^6 cells","offer_id":53362085757293,"sku":"TRC-0002-6C","price":1695.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ADCC-jurkat-flow.jpg?v=1776934273","url":"https:\/\/www.ebiohippo.com\/products\/cd16a-nfat-reporter-lentivirus-adcc-assay-bhv19400252","provider":"BioHippo","version":"1.0","type":"link"}