{"product_id":"cd24-antibody-bha17136295","title":"CD24 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCD24 Antibody is a anti-CD24 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as IHC-P with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD24\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCD24 antibody detects Cluster of differentiation 24, a small glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein involved in cell adhesion, immune modulation, and cancer metastasis. Encoded by the CD24 gene on chromosome 6q21, this protein is expressed on developing hematopoietic cells, mature B cells, granulocytes, and various epithelial and neuronal tissues. CD24 mediates cell-cell and cell-matrix interactions by binding to P-selectin and Siglec family receptors, influencing immune cell trafficking and signaling.\u003cbr\u003e\u003cbr\u003eStructurally, CD24 is a heavily glycosylated protein lacking a transmembrane domain, anchored to the plasma membrane via a GPI linkage. The carbohydrate moieties of CD24 contribute to its interactions with lectins and adhesion molecules, which regulate lymphocyte homing and activation. In the nervous system, CD24 is involved in axon growth and synaptic plasticity, while in the immune system it acts as a costimulatory molecule modulating T cell responses. CD24 engagement can influence B cell maturation, dendritic cell activation, and apoptosis signaling.\u003cbr\u003e\u003cbr\u003eThe CD24 antibody is widely used in immunology, oncology, and stem cell biology research to study immune signaling, cancer progression, and differentiation. Flow cytometry using this antibody identifies CD24-positive cell populations, while western blot analysis detects the mature glycoprotein at approximately 35-40 kilodaltons, with size variability due to glycosylation. Immunohistochemistry reveals membrane staining in lymphoid tissues and various carcinomas.\u003cbr\u003e\u003cbr\u003eIn cancer research, CD24 has emerged as a key marker of tumor-initiating cells and metastasis. Overexpression is associated with aggressive tumor phenotypes in breast, ovarian, and colorectal cancers, where CD24 promotes cell adhesion to P-selectin on platelets and endothelial cells, aiding metastatic spread. Conversely, CD24 expression can modulate immune responses by interacting with Siglec-10, inhibiting innate immune activation. The CD24 antibody is an essential tool for investigating immune regulation, tumor biology, and cell surface glycoprotein signaling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD24 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD24 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD24 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD24 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD24+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047324934509,"sku":"RQ8876","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f450525e-185c-4ec9-8357-88148b92fb39.jpg?v=1772019441","url":"https:\/\/www.ebiohippo.com\/products\/cd24-antibody-bha17136295","provider":"BioHippo","version":"1.0","type":"link"}