{"product_id":"cd31-antibody-pecam-1-bha17128445","title":"CD31 Antibody \/ PECAM-1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eCD31 Antibody \/ PECAM-1 is an antibody targeting \u003cstrong\u003ePECAM-1\u003c\/strong\u003e, raised in \u003cstrong\u003eGoat\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PECAM-1.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (goat origin); Goat Ig.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e IF, IHC-P, ELISA (peptide) (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eCD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a transmembrane glycoprotein that is found on the surface of various cell types, including platelets, endothelial cells, and leukocytes. One of the key roles of CD31 is its involvement in leukocyte trafficking and adhesion. Studies have shown that CD31 plays a crucial role in regulating the migration of immune cells to sites of inflammation or infection. By interacting with other proteins on the surface of immune cells, CD31 helps to mediate the adhesion and transmigration of these cells across the endothelium, allowing them to reach their target tissues and carry out their immune functions. CD31 has also been shown to regulate other important immune responses. For example, CD31 has been implicated in the modulation of T cell activation and proliferation. By interacting with other signaling molecules, CD31 can influence the signaling pathways that control T cell activation, ultimately shaping the immune response to pathogens or foreign antigens. Additionally, CD31 plays a role in the maintenance of vascular integrity. By promoting interactions between endothelial cells, CD31 helps to maintain the tight junctions that form the blood-brain barrier and other vascular structures. This function of CD31 is crucial for preventing the entry of pathogens and toxins into the bloodstream, protecting the body from potential harm.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eImmunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eImmunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003cli\u003eELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProtKB entry P16284 — UniProt — https:\/\/www.uniprot.org\/uniprotkb\/P16284 - Gene search: PECAM-1 — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PECAM-1 - Ensembl search: PECAM-1 — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PECAM-1 - PubMed search: PECAM-1 antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PECAM-1+antibody - Reactome search: PECAM-1 — Reactome — https:\/\/reactome.org\/content\/query?q=PECAM-1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5 mg\/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide \/ 100 ug","offer_id":53046421094765,"sku":"R36497-100UG","price":475.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_545a2e8b-9e10-45db-8dea-c6888ba724c2.jpg?v=1772000539","url":"https:\/\/www.ebiohippo.com\/products\/cd31-antibody-pecam-1-bha17128445","provider":"BioHippo","version":"1.0","type":"link"}