{"product_id":"cd40-nf-kappa-b-reporter-lentivirus-bhv19400263","title":"CD40\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCD40 is a cell surface receptor of the tumor necrosis factor receptor superfamily, expressed prominently on antigen-presenting cells such as B cells, macrophages, and dendritic cells. Engagement of CD40 by its ligand CD40L, expressed on activated T cells, delivers a critical co-stimulatory signal that licenses antigen-presenting cells, promotes B cell proliferation and antibody class switching, and enhances dendritic cell maturation. CD40 signaling proceeds through TRAF adaptor proteins to activate the transcription factor NF-κB, driving expression of genes that coordinate adaptive immune responses. CD40 is studied for its central role in T cell-dependent immunity and is an actively pursued target for cancer immunotherapy and vaccine development.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CD40\/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human CD40 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable cell line that responds quantitatively to CD40 engagement with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antigen-presenting cell function, immune regulation, and CD40-targeting therapeutics in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human CD40 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616276845,"sku":"TRV-0014-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_765708a9-0cf1-4f10-9dc5-77332fa77464.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/cd40-nf-kappa-b-reporter-lentivirus-bhv19400263","provider":"BioHippo","version":"1.0","type":"link"}