{"product_id":"cd63-antibody-bha17135299","title":"Cd63 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCd63 Antibody is a anti-CD63 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CD63\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe CD63 antibody targets CD63 antigen, a tetraspanin family member encoded by the CD63 gene that is widely expressed on cell membranes and intracellular vesicles. CD63 plays critical roles in vesicle trafficking, exosome biogenesis, and cellular adhesion. As a component of late endosomes and lysosomes, it participates in membrane fusion events and exocytosis regulation. The CD63 antibody provides researchers with a reliable marker for exosomes, multivesicular bodies, and activated platelets.\u003cbr\u003e\u003cbr\u003eCD63, also known as lysosomal-associated membrane protein 3, contains four transmembrane domains and two extracellular loops that mediate interactions with integrins and other tetraspanins. The CD63 antibody enables visualization of this protein on endosomal and plasma membranes, supporting studies of vesicle dynamics and signal transduction. In immune cells, CD63 is upregulated upon activation and translocated to the surface, where it modulates antigen presentation and cell adhesion processes.\u003cbr\u003e\u003cbr\u003eIn exosome biology, CD63 serves as a canonical marker used to identify and isolate extracellular vesicles derived from endosomal compartments. The CD63 antibody supports flow cytometry, immunoblotting, and immunoelectron microscopy applications to confirm exosome purity and origin. Its detection on vesicular membranes has made it an essential reagent in extracellular vesicle research. CD63 also interacts with proteins such as CD9 and CD81, forming tetraspanin-enriched microdomains that coordinate signaling receptor organization.\u003cbr\u003e\u003cbr\u003eClinically, altered CD63 expression has been associated with cancer progression, immune disorders, and allergic responses. Tumor-derived exosomes enriched in CD63 contribute to intercellular communication that promotes metastasis and immune evasion. The CD63 antibody allows quantitative and localization-based analysis of these vesicles in plasma or tissue samples. In platelets, CD63 acts as a marker of degranulation and activation, supporting research into coagulation and vascular inflammation.\u003cbr\u003e\u003cbr\u003eThe CD63 antibody performs strongly in western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. It provides distinct membrane and vesicular staining in multiple cell types.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CD63 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CD63 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CD63 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CD63 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CD63+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286792557,"sku":"FY12396","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bd301257-0156-44c2-8b94-b491952eea7d.jpg?v=1782236990","url":"https:\/\/www.ebiohippo.com\/products\/cd63-antibody-bha17135299","provider":"BioHippo","version":"1.0","type":"link"}