{"product_id":"cd8-positive-t-cells-negatively-selected-human-bhc18200211","title":"CD8+ T cells, Negatively Selected (Human)","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table tr:nth-child(even) th{background:#dce8fb}\n.bhp-desc .bhp-spec-table tr:nth-child(even) td{background:#f0f6ff}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:10px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444;margin:10px 0 14px}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eImmune Cell Markers\u003c\/span\u003e\n\u003cp\u003eCryopreserved vial (10 x 10^6 cells) of CD8+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD4+ T cells, gamma\/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD8+ T cells via immunomagnetic separation. Before and after CD8+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate. Source\u003cbr\u003eNormal human PBMC from Leukapheresis Sample\u003c\/p\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHarmonized Tariff Code\u003c\/th\u003e\n\u003ctd\u003e3002-5900\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003eHandling Directions\u003cbr\u003eFirst, pre-warm Immuno Culture Medium to 37°C. It is important to work quickly in the following\u003cbr\u003esteps to ensure high cell viability and recovery. Quickly thaw cells in a 37°C water bath with\u003cbr\u003econstant but gentle agitation. Clean the outside of the vial with 70% ethanol and immediately\u003cbr\u003etransfer the entire contents to a 50 ml tube. Slowly add 10 ml of pre-warmed medium while gently\u003cbr\u003eswirling the tube to mix. Centrifuge the cell suspension at 300 x g for 15 minutes at room\u003cbr\u003etemperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently\u003cbr\u003eresuspend the cell pellet by flicking the tube, then add the desired volume of warm medium, and\u003cbr\u003emix. NOTE: Up to 30% cell loss can be expected during washing steps. Cells are now ready for\u003cbr\u003euse in downstream applications.\u003c\/p\u003e\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLicense Required\u003c\/strong\u003eNo\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLiving Modified Organism\u003c\/strong\u003eNo\/not applicable (no new genetic material)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eCharacterization Criteria\u003cbr\u003eCell count, viability (trypan blue exclusion and FACS with impermeable DNA binding dye), and surface expression of CD3 and CD4\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated Products: Cat. #90184-B, 90184-A, 79704, 79752\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"10 million cells","offer_id":53322321690989,"sku":"79753","price":795.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/79753_img.jpg?v=1778636472","url":"https:\/\/www.ebiohippo.com\/products\/cd8-positive-t-cells-negatively-selected-human-bhc18200211","provider":"BioHippo","version":"1.0","type":"link"}