{"product_id":"cdc25a-phospho-s124-polyclonal-antibody-bha11302619","title":"CDC25A (Phospho-S124) polyclonal antibody","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n  \u003cspan class=\"bhp-cat-pill\"\u003ePrimary Antibody — Polyclonal\u003c\/span\u003e\n\n  \u003ch2\u003eProduct Overview\u003c\/h2\u003e\n  \u003cp\u003ep-CDC25A (S124) polyclonal antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Ser124.\u003c\/p\u003e\n\n  \u003cp\u003eCyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle.\u003c\/p\u003e\n\n  \u003ch2\u003eValidated Applications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eWestern Blot (WB)\u003c\/td\u003e\n\u003ctd\u003e1:500~1:1000\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/table\u003e\n\n  \u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eRabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003e~ 59,70 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eModification\u003c\/th\u003e\n\u003ctd\u003ePhosphorylated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt \/ Swiss-Prot\u003c\/th\u003e\n\u003ctd\u003eP30304\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eSignaling Pathway(s)\u003c\/th\u003e\n\u003ctd\u003eCell Cycle Control G1 S Checkpoint; G Protein-coupled Receptors Signaling to MAPK Erk; Cell Cycle G2 M DNA Damage Signaling Pathway\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n  \u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n  \u003cp\u003eSynthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of Serine 124.\u003c\/p\u003e\n\n  \u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n  \u003cdiv class=\"bhp-warning-box\"\u003e\n    \u003cp\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use. \n    Handle as a potential biohazard in accordance with good laboratory practices.\u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003ch2\u003eRelated Products\u003c\/h2\u003e\n  \u003cp\u003eExplore complementary antibodies, ELISA kits, and detection reagents at \n  \u003ca href=\"https:\/\/www.ebiohippo.com\" target=\"_blank\"\u003eBioHippo\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"50 uL","offer_id":53328198599021,"sku":"BS4668-50UL","price":168.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53328292053357,"sku":"BS4668-100UL","price":298.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nopic_2569f9d5-64cb-4fa4-9b8c-48a580dc5243.jpg?v=1778730540","url":"https:\/\/www.ebiohippo.com\/products\/cdc25a-phospho-s124-polyclonal-antibody-bha11302619","provider":"BioHippo","version":"1.0","type":"link"}