{"product_id":"cdc73-antibody-cell-division-cycle-protein-73-bha17135481","title":"CDC73 Antibody \/ Cell division cycle protein 73","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCDC73 Antibody \/ Cell division cycle protein 73 is a anti-CDC73 Rabbit antibody Recombinant Rabbit Monoclonal clone 31C54 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CDC73\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31C54, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCDC73 antibody detects cell division cycle protein 73, a tumor suppressor encoded by the CDC73 gene. CDC73 is also known as parafibromin and functions as a subunit of the PAF1 transcriptional regulatory complex. Through its association with RNA polymerase II, CDC73 regulates transcription elongation, histone modification, and RNA processing. Its tumor suppressor activity is critical for preventing uncontrolled cell growth and maintaining genomic integrity.\u003cbr\u003e\u003cbr\u003eCDC73 antibody is widely applied in cancer research, molecular biology, and genetic studies. Mutations in CDC73 are associated with hyperparathyroidism-jaw tumor syndrome and parathyroid carcinoma. By detecting CDC73 expression, researchers can investigate its role in tumor suppression and transcriptional control. Loss of CDC73 expression serves as a diagnostic marker for parathyroid malignancy and supports studies of endocrine tumors.\u003cbr\u003e\u003cbr\u003e Western blot assays reveal CDC73 protein in nuclear extracts, while immunohistochemistry highlights nuclear localization in normal tissues and its loss in tumor samples. Immunofluorescence provides high-resolution visualization of parafibromin within nuclear transcriptional complexes. These applications make CDC73 antibody valuable for both research and diagnostic contexts.\u003cbr\u003e\u003cbr\u003eCDC73 also regulates histone methylation and chromatin remodeling through its interaction with the PAF1 complex. This regulation influences expression of genes involved in cell cycle, apoptosis, and development. By applying CDC73 antibody, scientists can study how transcriptional regulation by parafibromin contributes to normal growth and cancer prevention.\u003cbr\u003e\u003cbr\u003eCDC73 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CDC73 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CDC73 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CDC73 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CDC73 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CDC73+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047292952941,"sku":"FY12578","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1bb96f00-fbee-43fc-a541-04b00b56bc1a.jpg?v=1772019311","url":"https:\/\/www.ebiohippo.com\/products\/cdc73-antibody-cell-division-cycle-protein-73-bha17135481","provider":"BioHippo","version":"1.0","type":"link"}