{"product_id":"cdh1-antibody-e-cadherin-bha17136020","title":"Cdh1 Antibody \/ E-cadherin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCdh1 Antibody \/ E-cadherin is a anti-CDH1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CDH1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCdh1 antibody detects E-cadherin, a calcium-dependent cell adhesion glycoprotein critical for epithelial cell-cell junctions and tissue integrity. The UniProt recommended name is Cadherin-1 (CDH1), also known as E-cadherin. This transmembrane protein mediates homophilic interactions between epithelial cells, maintaining polarity and barrier function across tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, E-cadherin antibody identifies a 882-amino-acid protein composed of five extracellular cadherin repeats, a transmembrane segment, and a cytoplasmic domain that binds catenins. Through its linkage to beta-catenin and the actin cytoskeleton, E-cadherin forms adherens junctions that regulate tissue morphogenesis and cell migration. Its adhesive strength depends on calcium ions that stabilize the extracellular cadherin domains.\u003cbr\u003e\u003cbr\u003eThe CDH1 gene is located on chromosome 16q22.1 and is strongly expressed in epithelial tissues, including gastrointestinal tract, liver, mammary gland, and skin. E-cadherin serves as a hallmark of epithelial differentiation, while its downregulation signifies epithelial-to-mesenchymal transition (EMT), a key event in cancer metastasis and wound repair.\u003cbr\u003e\u003cbr\u003ePathologically, loss or mutation of E-cadherin leads to disrupted cell adhesion, increased invasiveness, and hereditary diffuse gastric cancer. CDH1 promoter methylation and transcriptional repression by SNAIL or TWIST contribute to tumor progression. Research using E-cadherin antibody supports studies in cell adhesion, cancer metastasis, and epithelial polarity.\u003cbr\u003e\u003cbr\u003eCdh1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect E-cadherin in tissues and cultured epithelial cells. \u003cbr\u003e\u003cbr\u003eStructurally, E-cadherin forms cis and trans dimers that bridge adjacent cell membranes, with its cytoplasmic domain binding to catenins and actin filaments. This antibody facilitates detailed characterization of E-cadherin's role in maintaining epithelial integrity and suppressing invasion.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CDH1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CDH1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CDH1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CDH1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CDH1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312515437,"sku":"FY13118","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_64d560d5-29d0-4268-b4eb-8991bdbde6d5.jpg?v=1782237109","url":"https:\/\/www.ebiohippo.com\/products\/cdh1-antibody-e-cadherin-bha17136020","provider":"BioHippo","version":"1.0","type":"link"}