{"product_id":"cell-cycle-reporter-lentivirus-bhv19400048","title":"Cell Cycle Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCell cycle progression through the G1, S, G2, and M phases is tightly coordinated to ensure faithful DNA replication and division. A key mechanism of phase control is regulated protein degradation: the licensing factor CDT1 accumulates in G1 and is degraded as cells enter S phase, while Geminin accumulates during S, G2, and M and is degraded at the end of mitosis. The fluorescent ubiquitination-based cell cycle indicator (FUCCI) exploits these reciprocal oscillations by fusing fluorescent proteins to fragments of CDT1 and Geminin, allowing real-time visualization of cell cycle phase in living cells. This approach is widely used in studies of proliferation, differentiation, stem cell biology, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Cell Cycle Reporter Lentivirus delivers a FUCCI-based system for visualizing cell cycle progression in living cells without fixation. Human CDT1 (amino acids 30-120), which accumulates in G1, is fused to RFP, and human Geminin (amino acids 1-110), which accumulates in G2\/M, is fused to GFP. A CMV promoter drives RFP-CDT1, GFP-Geminin, and a drug selection marker as a single transcript, with self-cleaving T2A and P2A sequences yielding three separate proteins for balanced reporter expression.\u003c\/p\u003e\n\u003cp\u003eThis arrangement enables precise monitoring of all cell cycle phases in stable or transiently transduced cells. Supplied as VSV-G-pseudotyped particles purified by PEG precipitation and sucrose gradient centrifugation, the product suits a wide range of mammalian cells, including primary, thawed, and pluripotent stem cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, GFP, hCDT1-RFP + hGemn-GFP, Luc, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the Cell cycle (phases) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251615326573,"sku":"LTV-0052-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53251635347821,"sku":"LTV-0052-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FUCCI-Puro-Fig.1.jpg?v=1776934330","url":"https:\/\/www.ebiohippo.com\/products\/cell-cycle-reporter-lentivirus-bhv19400048","provider":"BioHippo","version":"1.0","type":"link"}