{"product_id":"celltrypase-c-lecta-bhz16500003","title":"CellTrypase – Recombinant Trypsin-Like Enzyme for Cell Dissociation","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc ul{margin:0 0 14px;padding-left:20px}\n.bhp-desc li{margin-bottom:6px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:12px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 12px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eTrypsin-like serine proteases catalyze peptide bond cleavage at the carboxyl side of lysine and arginine residues — the same enzymatic mechanism exploited during cell dissociation (trypsinization). Conventional porcine trypsin introduces biosafety concerns associated with animal-derived materials, including batch-to-batch variability and potential adventitious agent contamination. Recombinant, non-mammalian alternatives address these risks by enabling consistent, animal origin-free production under controlled manufacturing conditions.\u003c\/p\u003e\n\u003cp\u003eCellTrypase is a recombinant trypsin-like enzyme derived from \u003cem\u003eFusarium oxysporum\u003c\/em\u003e, produced via microbial fermentation using \u003cem\u003eBacillus\u003c\/em\u003e sp. as the expression host. Its fungal origin confers inherently lower non-specific reactivity compared to mammalian trypsin, resulting in gentler cell dissociation with preserved viability and function.\u003c\/p\u003e\n\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eExpression System: Microbial Fermentation (Bacillus sp.)\u003c\/span\u003e\n\u003cp\u003eCellTrypase is manufactured in-house by c-LEcta GmbH (a Kerry Company) under a certified Quality Management System. It is available in two quality grades — GMP-grade (EXCiPACT® GMP\/GDP certified) and R\u0026amp;D-grade (ISO 9001 compliant) — and in two concentration formats (1x ready-to-use; 10x concentrated stock), supplied in 100 mL and 500 mL bottle sizes.\u003c\/p\u003e\n\u003cp\u003eThe formulation is a sterile-filtered liquid in PBS with EDTA. By eliminating drying and reconstitution steps, the liquid format minimizes degradation risk and preserves enzyme integrity — yielding a crystal-clear solution that visibly reflects its purity.\u003c\/p\u003e\n\u003cdiv class=\"bhp-qc-badge\"\u003e✔ GMP-Grade Available | EXCiPACT® Certified | Animal Origin-Free\u003c\/div\u003e\n\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme Origin\u003c\/th\u003e\n\u003ctd\u003eRecombinant \u003cem\u003eFusarium oxysporum\u003c\/em\u003e expressed in \u003cem\u003eBacillus\u003c\/em\u003e sp.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzyme Class\u003c\/th\u003e\n\u003ctd\u003eSerine Protease (Trypsin-Like)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCleavage Specificity\u003c\/th\u003e\n\u003ctd\u003eCarboxyl side of Lys and Arg residues\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLiquid in PBS with EDTA, sterile filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAppearance\u003c\/th\u003e\n\u003ctd\u003eClear, transparent to slightly colored solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e≥95% (determined by HPLC — identity confirmed)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivity (1x)\u003c\/th\u003e\n\u003ctd\u003e0.6–1.2 kU\/L (photometric; 37 °C, 8 mM L-AAPA, pH 8.0; 1 U = 1 μmol \u003cem\u003ep\u003c\/em\u003e-nitroaniline\/min)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivity (10x)\u003c\/th\u003e\n\u003ctd\u003e6–12 kU\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003epH\u003c\/th\u003e\n\u003ctd\u003e7.1–7.6 (Ph. Eur. 2.2.3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOsmolality\u003c\/th\u003e\n\u003ctd\u003e270–320 mOsm\/kg (Ph. Eur. 2.2.35)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level (1x)\u003c\/th\u003e\n\u003ctd\u003e≤1 EU\/mL (LAL-Test; Ph. Eur. 2.6.14 \/ USP \u0026lt;85\u0026gt; Method D)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level (10x)\u003c\/th\u003e\n\u003ctd\u003e≤10 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMycoplasma Testing\u003c\/th\u003e\n\u003ctd\u003eNegative (qPCR Detection Assay; Ph. Eur. 2.6.7)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSterility\u003c\/th\u003e\n\u003ctd\u003ePass (Ph. Eur. 2.6.1 \/ USP \u0026lt;71\u0026gt;)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e2–8 °C ±5 °C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eAmbient temperature (stability data confirmed for ≥6 months)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAvailable Sizes\u003c\/th\u003e\n\u003ctd\u003e100 mL · 500 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eQuality Grades\u003c\/th\u003e\n\u003ctd\u003eGMP-grade (EXCiPACT® GMP\/GDP) · R\u0026amp;D-grade (ISO 9001)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration Formats\u003c\/th\u003e\n\u003ctd\u003e1x ready-to-use · 10x concentrated stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal Origin-Free\u003c\/th\u003e\n\u003ctd\u003eYes — raw materials, equipment, and packaging AOF\/TSE\/BSE certified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibiotic-Free\u003c\/th\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePackaging\u003c\/th\u003e\n\u003ctd\u003eNon-pyrogenic PET containers, HDPE screw caps; tamper-evident shrink seal (USP Class VI \/ 21 CFR compliant)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eValidated Performance\u003c\/h2\u003e\n\u003cp\u003eCellTrypase has been validated across key biopharma production cell lines for viral vector and vaccine manufacturing, demonstrating efficient trypsinization with high cell viability and robust recovery. The enzyme shows dissociation kinetics, cell replating efficiency, proliferation, and long-term culture maintenance comparable to porcine trypsin and existing recombinant alternatives.\u003c\/p\u003e\n\u003cp\u003eCellTrypase supports direct 1:1 substitution in existing trypsinization protocols. Validated applications include:\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003ePassaging and expansion of adherent cell lines (e.g., Vero, MDCK, HEK293)\u003c\/li\u003e\n  \u003cli\u003eCell dissociation in viral vector and vaccine production for cell and gene therapies\u003c\/li\u003e\n  \u003cli\u003eStem cell processing: iPSCs, hESCs, and organoids\u003c\/li\u003e\n  \u003cli\u003ePrimary cell isolation: fibroblasts, keratinocytes, chondrocytes, neuronal cells\u003c\/li\u003e\n  \u003cli\u003eCell banking and tissue engineering workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003ePurity\u003c\/strong\u003e≥95% HPLC\u003c\/div\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eQMS (GMP)\u003c\/strong\u003eEXCiPACT® GMP\/GDP\u003c\/div\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eQMS (R\u0026amp;D)\u003c\/strong\u003eISO 9001\u003c\/div\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eMycoplasma\u003c\/strong\u003eTested Negative\u003c\/div\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eSterility\u003c\/strong\u003ePass (Ph. Eur.\/USP)\u003c\/div\u003e\n  \u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eEndotoxin\u003c\/strong\u003eLAL-Tested\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eInactivation \u0026amp; Handling Protocol\u003c\/h2\u003e\n\u003cp\u003eCellTrypase requires no special inhibitor for inactivation. After cell detachment, quench activity by adding fresh medium (serum-free or serum-containing). For complete enzyme removal, centrifuge and resuspend cells in fresh medium.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQuick Protocol (Adherent Cell Dissociation):\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003ePre-warm enzyme and medium to 37 °C\u003c\/li\u003e\n  \u003cli\u003eRinse cells with PBS (Ca²⁺\/Mg²⁺-free)\u003c\/li\u003e\n  \u003cli\u003eAdd CellTrypase (~1 mL per 25 cm²)\u003c\/li\u003e\n  \u003cli\u003eIncubate at 37 °C (or room temperature) until detachment\u003c\/li\u003e\n  \u003cli\u003eQuench with fresh medium; centrifuge if required\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). GMP-grade material is intended for use as a processing aid in biopharmaceutical manufacturing. Not for direct administration to humans or animals.\u003c\/div\u003e\n\n\u003ch2\u003eRegulatory Support \u0026amp; Documentation\u003c\/h2\u003e\n\u003cp\u003ec-LEcta provides comprehensive documentation to support customer qualification, validation, and regulatory submissions, including: Certificate of Analysis (CoA), Safety Data Sheet (SDS), animal origin-free declarations, TSE\/BSE statements, packaging details, change notifications, and quality agreements. A Validation Guide and Application Note for key biopharma cell lines are available upon request. A Drug Master File (DMF) submission is part of the regulatory roadmap for future global filings (EMA, FDA, ANSM).\u003c\/p\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eThis product is manufactured and distributed by c-LEcta GmbH, a Kerry Company (Leipzig, Germany). Regulatory and quality documentation is available for GMP-grade orders. Free 100 mL samples are available for qualification and process development testing.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"c-LEcta GmbH","offers":[{"title":"R\u0026D Grade \/ 1x (Ready-to-Use) \/ 100 mL","offer_id":53379624829293,"sku":"22103-1X-100","price":32.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 1x (Ready-to-Use) \/ 500 mL","offer_id":53379625091437,"sku":"22103-1X-500","price":110.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 10x (Concentrated Stock) \/ 100 mL","offer_id":53379625124205,"sku":"22103-10X-100","price":104.0,"currency_code":"USD","in_stock":true},{"title":"R\u0026D Grade \/ 10x (Concentrated Stock) \/ 500 mL","offer_id":53379625156973,"sku":"22103-10X-500","price":451.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 1x (Ready-to-Use) \/ 100 mL","offer_id":53379625189741,"sku":"22103-1X-100-G","price":42.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 1x (Ready-to-Use) \/ 500 mL","offer_id":53379625222509,"sku":"22103-1X-500-G","price":146.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 10x (Concentrated Stock) \/ 100 mL","offer_id":53379625255277,"sku":"22103-10X-100-G","price":138.0,"currency_code":"USD","in_stock":true},{"title":"GMP Grade \/ 10x (Concentrated Stock) \/ 500 mL","offer_id":53379625288045,"sku":"22103-10X-500-G","price":602.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CellTrypase_Products_20Packaging.jpg?v=1779996972","url":"https:\/\/www.ebiohippo.com\/products\/celltrypase-c-lecta-bhz16500003","provider":"BioHippo","version":"1.0","type":"link"}