{"product_id":"cep55-antibody-centrosomal-protein-of-55-bha17135386","title":"CEP55 Antibody \/ Centrosomal protein of 55","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCEP55 Antibody \/ Centrosomal protein of 55 is a anti-CEP55 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CEP55\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCEP55 antibody recognizes Centrosomal protein of 55 kilodaltons, a key regulator of cytokinesis and abscission during cell division. CEP55 is essential for recruiting the ESCRT (endosomal sorting complex required for transport) machinery that mediates final membrane separation between daughter cells. The CEP55 antibody is widely used in cell cycle and cancer research to study centrosome function, mitotic exit, and cytokinetic regulation.\u003cbr\u003e\u003cbr\u003eCEP55 is encoded by the CEP55 gene on human chromosome 10q23.33. The protein localizes to the centrosome and midbody during late mitosis, coordinating the final stages of cytokinesis. Structurally, CEP55 contains coiled-coil domains and a C-terminal ESCRT- and ALIX-binding region that enables its interaction with ESCRT-I component TSG101 and adaptor ALIX (PDCD6IP). These interactions recruit membrane scission complexes to the midbody, ensuring successful separation of daughter cells following chromosomal segregation.\u003cbr\u003e\u003cbr\u003eThe CEP55 antibody identifies a 55-60 kilodalton protein in western blot analysis, while immunofluorescence reveals strong midbody and centrosomal staining during telophase and cytokinesis. CEP55 overexpression is frequently observed in tumors, including breast, colon, and lung cancers, where it promotes uncontrolled proliferation and genomic instability. As a consequence, CEP55 is considered both a mitotic marker and a potential therapeutic target in oncology. In normal physiology, it functions as a checkpoint component ensuring that abscission only occurs after proper chromosomal segregation and spindle formation.\u003cbr\u003e\u003cbr\u003eFunctionally, CEP55 is phosphorylated by PLK1 and Aurora B kinases during mitosis, and these phosphorylation events regulate its recruitment timing to the midbody. Disruption of CEP55 impairs ESCRT recruitment, leading to cytokinesis failure and multinucleated cell formation. Beyond cytokinesis, CEP55 contributes to cilia disassembly and may play a role in signal transduction during cell cycle re-entry.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CEP55 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CEP55 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CEP55 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CEP55 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CEP55+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290495341,"sku":"FY12483","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_aed54bc4-4e2e-4699-a9fd-29eec944cfb2.jpg?v=1782237016","url":"https:\/\/www.ebiohippo.com\/products\/cep55-antibody-centrosomal-protein-of-55-bha17135386","provider":"BioHippo","version":"1.0","type":"link"}