{"product_id":"chd4-antibody-chromodomain-helicase-dna-binding-protein-4-mi2-beta-bha17136231","title":"CHD4 Antibody \/ Chromodomain-helicase-DNA-binding protein 4 \/ Mi2-beta","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCHD4 Antibody \/ Chromodomain-helicase-DNA-binding protein 4 \/ Mi2-beta is a anti-CHD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CHD4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCHD4 antibody detects Chromodomain-helicase-DNA-binding protein 4, also called Mi2-beta, a nuclear ATP-dependent chromatin remodeler encoded by the CHD4 gene on chromosome 12p13.31. CHD4 is a member of the CHD family (chromodomain helicase DNA-binding proteins) and serves as the catalytic core of the nucleosome remodeling and deacetylase (NuRD) complex. This complex couples ATP-dependent chromatin remodeling with histone deacetylation to regulate gene transcription, DNA repair, and replication. CHD4 is highly expressed in proliferating cells, hematopoietic lineages, and neurons, where it orchestrates transcriptional repression and chromatin organization.\u003cbr\u003e\u003cbr\u003eAs a key nuclear regulator, CHD4 modulates chromatin accessibility and transcriptional silencing by repositioning nucleosomes and recruiting histone-modifying enzymes. It interacts with co-repressors, including MTA1-3 and HDAC1\/2, within the NuRD complex to establish repressive chromatin states. CHD4's helicase and chromodomain motifs enable recognition of methylated histones and remodeling of nucleosomal DNA, linking epigenetic signals to transcriptional control. It functions in diverse pathways such as cell cycle progression, stem cell maintenance, and DNA damage repair.\u003cbr\u003e\u003cbr\u003eCHD4 antibody identifies a protein that interacts with PARP1 and BRCA1 at sites of DNA double-strand breaks, coordinating chromatin remodeling and homologous recombination. Through this association, CHD4 contributes to the DNA damage response (DDR), facilitating repair factor recruitment and checkpoint activation. Mutations or misregulation of CHD4 impair genomic stability and can lead to carcinogenesis or developmental disorders. CHD4 also associates with replication protein A (RPA) and proliferating cell nuclear antigen (PCNA), supporting chromatin reassembly during DNA synthesis.\u003cbr\u003e\u003cbr\u003eStructurally, CHD4 contains two N-terminal plant homeodomain (PHD) zinc fingers, tandem chromodomains for methylated histone recognition, an ATPase\/helicase domain for chromatin remodeling, and a C-terminal region mediating protein-protein interactions. This modular structure allows CHD4 to integrate epigenetic marks with mechanical chromatin rearrangement. It is part of the CHD family of SNF2-like ATPases that also includes CHD3 and CHD5, all involved in transcriptional regulation and chromatin remodeling.\u003cbr\u003e\u003cbr\u003eDysfunction of CHD4 is linked to various diseases. Somatic mutations have been identified in endometrial carcinoma, chronic lymphocytic leukemia, and glioblastoma. Germline CHD4 mutations cause Sifrim-Hitz-Weiss syndrome, characterized by developmental delay, craniofacial abnormalities, and cardiac defects. Pathway analysis places CHD4 in the chromatin remodeling, DNA repair, and cell cycle regulation pathways. It also co-localizes with HDAC1\/2 and MTA proteins in nuclear repressor complexes, reinforcing its central role in chromatin dynamics.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using CHD4 antibody shows strong nuclear localization in proliferating and stem-like cells. The CHD4 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CHD4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CHD4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CHD4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CHD4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CHD4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323427181,"sku":"FY13329","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_585031c5-42f5-40f2-9f41-3f3c77ff61b4.jpg?v=1782237100","url":"https:\/\/www.ebiohippo.com\/products\/chd4-antibody-chromodomain-helicase-dna-binding-protein-4-mi2-beta-bha17136231","provider":"BioHippo","version":"1.0","type":"link"}