{"product_id":"chicken-ibdv-antibody-elisa-kit-bhe21400553","title":"Chicken IBDV Antibody ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Chicken IBDV Antibody ELISA Kit detects antibodies against Chicken IBDV in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eQualitative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eELISA Microtiter plate\u003c\/td\u003e\n\u003ctd\u003e96 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e101×Concentrated Antibody Working Solution\u003c\/td\u003e\n\u003ctd\u003e110 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10×Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAntibody Working Buffer\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent A\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent B\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive Control\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNegative Control\u003c\/td\u003e\n\u003ctd\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e3 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSealed Bag\u003c\/td\u003e\n\u003ctd\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure\u003c\/h2\u003e\n\u003cp\u003eBring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eNumber the sample and control in order (in duplicate), and keep a record of control wells and sample wells. Set 1 well for negative\/positive control and 1 well for blank control. Run samples in duplicate.\u003c\/li\u003e\n\u003cli\u003eAdd 10 µL of positive\/negative control to positive\/negative control well, add 10 µL of Serum to sample well. Then add 90 µL of Antibody Working Solution to each well, add only 100 µL of Antibody Working Solution to blank control.\u003c\/li\u003e\n\u003cli\u003eCover with a plate sealer and mix thoroughly, incubate at 37 °C for 30 min protected from light.\u003c\/li\u003e\n\u003cli\u003eRemove the liquid in each well. Immediately add 300 µL of Wash Buffer to each well and wash. Repeat 3 times, soaking 1 min each time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL of HRP Conjugate to each well, cover with a plate sealer and incubate at 37 °C for 30 min protected from light.\u003c\/li\u003e\n\u003cli\u003eRepeat step 4 for washing.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well, Cover with a plate sealer and mix thoroughly, incubate at 37 °C for 10 min protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well, mix thoroughly.\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance of each well by using a microplate reader with 450 nm wavelength (use 630 nm as reference wavelength). This step should be finished in 10 min after stop reaction. A blank well is not needed with dual-wavelength reading\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRequired but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm \/ 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.\u003c\/p\u003e\n\u003ch2\u003eResult Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eS\/N value = Sample OD\/Average of negative control ODs.\u003c\/li\u003e\n\u003cli\u003eNegative result: S\/N value ≥ 0.7\u003c\/li\u003e\n\u003cli\u003ePositive result: S\/N value \u0026lt; 0.7\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. Store the kit at 2-8 °C and do not freeze. Once opened, keep the plate at 2-8 °C and use it within 1 month. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980405768557,"sku":"VK127018-96T","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_6713a029-35e2-4cea-8a09-1660ee45998e.jpg?v=1794521012","url":"https:\/\/www.ebiohippo.com\/products\/chicken-ibdv-antibody-elisa-kit-bhe21400553","provider":"BioHippo","version":"1.0","type":"link"}