{"product_id":"chicken-il-12p40-interleukin-12-p40-elisa-kit-bhe15207962","title":"Chicken IL-12p40(Interleukin 12 p40) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eIL-12p40 (Interleukin 12 p40)\u003c\/strong\u003e is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.\u003c\/p\u003e\u003cp\u003eInterleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.\u003c\/p\u003e\u003cp\u003eQuantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eIL-12p40 (Interleukin 12 p40)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eChicken\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 5.9 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965912674669,"sku":"ELK2744-96T","price":764.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965912707437,"sku":"ELK2744-48T","price":535.6,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965912740205,"sku":"ELK2744-96TX5","price":3248.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_767bd913-2f19-4037-a6ed-166cf2a58c23.jpg?v=1771845305","url":"https:\/\/www.ebiohippo.com\/products\/chicken-il-12p40-interleukin-12-p40-elisa-kit-bhe15207962","provider":"BioHippo","version":"1.0","type":"link"}